Vascular Alterations Underlie Developmental Problems Manifested in Cloned Cattle before or after Birth

Autor(es)

Maiorka, Paulo Cesar ^[1] ; Favaron, Phelipe Oliveira ^[2] ; Mess, Andrea Maria ^[2] ; dos Santos, Caio Rodrigues ^[1] ; Alberto, Miryan Lanca ^[2] ; Meirelles, Flavio Vieira ^[3] ; Miglino, Maria Angelica ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Med, Dept Surg, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Anim Sci & Food Engn, Pirassununga - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 1 JAN 13 2015.
• Citações Web of Science: 6

Resumo

Although assisted reproductive techniques are commonly applied in humans and animals, they are frequently associated with major developmental deficits and reduced viability. To explore abnormalities associated with cloning or nuclear transfer (NT) as the most invasive of these methods, we used a bovine model to characterize abnormalities. Detailed necropsy examinations were done on 13 calves that died soon after birth; in addition, we included data from embryos and fetuses (produced by NT) that terminated prematurely. Bovine clones that survived until the neonatal period differed quantitatively and qualitatively from in-vivo-derived cattle. Although alterations affected a variety of organs (e.g. heart, lung and liver), there was a clear association with abberant vascular developmental during the early intrauterine phase. Therefore, we concluded that vascular problems were key alterations induced by cloning (presumably via epigenetic modifications). (AU)

• Processo FAPESP: 05/60606-3 - Patologia molecular: avaliação de alterações genômicas proteômicas e glicobiológicas em tecidos pela técnica do tissue array
• Beneficiário: Paulo César Maiorka
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores