Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats

Autor(es):Mostrar menos -

Albuquerque-Pontes, Gianna Moes ^[1] ; Vieira, Rodolfo de Paula ^{[2, 3]} ; Tomazoni, Shaiane Silva ^[4] ; Caires, Claudia Oliveira ^[1] ; Nemeth, Victoria ^[2] ; Vanin, Adriane Aver ^[2] ; Santos, Larissa Aline ^[2] ; Pinto, Henrique Dantas ^[2] ; Marcos, Rodrigo Labat ^[4] ; Bjordal, Jan Magnus ^{[5, 6]} ; Camillo de Carvalho, Paulo de Tarso ^{[2, 1]} ; Pinto Leal-Junior, Ernesto Cesar ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo - Brazil

^[2] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, BR-01504001 Sao Paulo - Brazil

^[3] Univ Nove Julho UNINOVE, Postgrad Program Med, BR-01504001 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Pharmacol, Lab Pharmacol & Expt Therapeut, BR-05508900 Sao Paulo, SP - Brazil

^[5] Univ Bergen, Fac Med & Dent, Dept Global Publ Hlth, Physiotherapy Res Grp, N-5020 Bergen - Norway

^[6] Bergen Univ Coll, Ctr Knowledge Based Practice, N-5009 Bergen - Norway

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 30, n. 1, p. 59-66, JAN 2015.
• Citações Web of Science: 44

Resumo

Modulation of cytochrome c oxidase activity has been pointed as a possible key mechanism for low-level laser therapy (LLLT) in unhealthy biological tissues. But recent studies by our research group with LLLT in healthy muscles before exercise found delayed skeletal muscle fatigue development and improved biochemical status in muscle tissue. Therefore, the aim of this study was to evaluate effects of different LLLT doses and wavelengths in cytochrome c oxidase activity in intact skeletal muscle. In this animal experiment, we irradiated the tibialis anterior muscle of rats with three different LLLT doses (1, 3, and 10 J) and wavelengths (660, 830, and 905 nm) with 50 mW power output. After irradiation, the analyses of cytochrome c oxidase expression by immunohistochemistry were analyzed at 5, 10, 30 min and at 1, 2, 12, and 24 h. Our results show that LLLT increased (p < 0.05) cytochrome c oxidase expression mainly with the following wavelengths and doses: 660 nm with 1 J, 830 nm with 3 J, and 905 nm with 1 J at all time points. We conclude that LLLT can increase cytochrome c oxidase activity in intact skeletal muscle and that it contributes to our understanding of how LLLT can enhance performance and protect skeletal muscles against fatigue development and tissue damage. Our findings also lead us to think that the combined use of different wavelengths at the same time can enhance LLLT effects in skeletal muscle performance and other conditions, and it can represent a therapeutic advantage in clinical settings. (AU)

• Processo FAPESP: 12/11385-8 - Efeito de diferentes doses, comprimentos de onda e intervalos de aplicação da laserterapia de baixa potência na atividade de citocromo c-oxidase em diferentes tipos de fibras musculares no músculo esquelético de ratos.
• Beneficiário: Gianna Móes Albuquerque Pontes
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/06832-5 - Laserterapia de baixa potência e diclofenaco sódico de uso tópico na lesão muscular induzida por contusão em ratos
• Beneficiário: Lúcio Frigo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/52404-0 - Laserterapia de baixa potência na fadiga muscular e recuperação muscular pós-exercício: parâmetros ideais de aplicação e efeitos em exercício de longa duração
• Beneficiário: Ernesto Cesar Pinto Leal Junior
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores