Low-level laser therapy in IL-1 beta, COX-2, and PGE2 modulation in partially injured Achilles tendon

Autor(es)

de Jesus, Julio Fernandes ^[1] ; Spadacci-Morena, Diva Denelle ^[2] ; dos Anjos Rabelo, Nayra Deise ^[3] ; Pinfildi, Carlos Eduardo ^[4] ; Fukuda, Thiago Yukio ^[5] ; Plapler, Helio ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed So Paulo UNIFESP, Interdisciplinary Surg Sci Program, BR-04021001 Sao Paulo - Brazil

^[2] Inst Butantan, Lab Pathophysiol, BR-05504900 Sao Paulo - Brazil

^[3] Univ Nove Julho UNINOVE, Rehabil Sci Program, BR-02112000 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, BR-04021001 Sao Paulo - Brazil

^[5] ISCMSP, BR-01221020 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 30, n. 1, p. 153-158, JAN 2015.
• Citações Web of Science: 14

Resumo

This study evaluated IL-1 beta, COX-2, and PGE2 modulation in partially injured Achilles tendons treated with low-level laser therapy (LLLT). Sixty-five male Wistar rats were used. Sixty were submitted to a direct injury on Achilles tendon and then distributed into six groups: LASER 1 (a single LLLT application), LASER 3 (three LLLT applications), and LASER 7 (seven LLLT applications) and Sham 1, 3, and 7 (the same injury but LLLT applications were simulated). The five remaining animals were allocated at control group (no procedure performed). LLLT (780 nm) was applied with 70 mW of mean power and 17.5 J/cm(2) of fluency for 10 s, once a day. The tendons were surgically removed and assessed immunohistochemically for IL-1 beta, COX-2, and PGE2. In comparisons with control (IL-1 beta: 100.5 +/- 92.5 / COX-2: 180.1 +/- 97.1 / PGE2: 187.8 +/- 128.8) IL-1 beta exhibited (mean +/- SD) near-normal level (p > 0.05) at LASER 3 (142.0 +/- 162.4). COX-2 and PGE2 exhibited near-normal levels (p > 0.05) at LASER 3 (COX-2: 176.9 +/- 75.4 / PGE2: 297.2 +/- 259.6) and LASER 7 (COX-2: 259.2 +/- 190.4 / PGE2: 587.1 +/- 409.7). LLLT decreased Achilles tendon's inflammatory process. (AU)

• Processo FAPESP: 11/00979-1 - Influência do laser de baixa intensidade na modulação da inflamação e no reparo tecidual do tendão calcâneo do rato após lesão parcial
• Beneficiário: Helio Plapler
• Modalidade de apoio: Auxílio à Pesquisa - Regular