Low-level laser therapy in IL-1 beta, COX-2, and PGE2 modulation in partially injured Achilles tendon

Author(s)

de Jesus, Julio Fernandes ^[1] ; Spadacci-Morena, Diva Denelle ^[2] ; dos Anjos Rabelo, Nayra Deise ^[3] ; Pinfildi, Carlos Eduardo ^[4] ; Fukuda, Thiago Yukio ^[5] ; Plapler, Helio ^[4]

Affiliation

^[1] Univ Fed So Paulo UNIFESP, Interdisciplinary Surg Sci Program, BR-04021001 Sao Paulo - Brazil

^[2] Inst Butantan, Lab Pathophysiol, BR-05504900 Sao Paulo - Brazil

^[3] Univ Nove Julho UNINOVE, Rehabil Sci Program, BR-02112000 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, BR-04021001 Sao Paulo - Brazil

^[5] ISCMSP, BR-01221020 Sao Paulo - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 30, n. 1, p. 153-158, JAN 2015.
• Web of Science Citations: 14

Abstract

This study evaluated IL-1 beta, COX-2, and PGE2 modulation in partially injured Achilles tendons treated with low-level laser therapy (LLLT). Sixty-five male Wistar rats were used. Sixty were submitted to a direct injury on Achilles tendon and then distributed into six groups: LASER 1 (a single LLLT application), LASER 3 (three LLLT applications), and LASER 7 (seven LLLT applications) and Sham 1, 3, and 7 (the same injury but LLLT applications were simulated). The five remaining animals were allocated at control group (no procedure performed). LLLT (780 nm) was applied with 70 mW of mean power and 17.5 J/cm(2) of fluency for 10 s, once a day. The tendons were surgically removed and assessed immunohistochemically for IL-1 beta, COX-2, and PGE2. In comparisons with control (IL-1 beta: 100.5 +/- 92.5 / COX-2: 180.1 +/- 97.1 / PGE2: 187.8 +/- 128.8) IL-1 beta exhibited (mean +/- SD) near-normal level (p > 0.05) at LASER 3 (142.0 +/- 162.4). COX-2 and PGE2 exhibited near-normal levels (p > 0.05) at LASER 3 (COX-2: 176.9 +/- 75.4 / PGE2: 297.2 +/- 259.6) and LASER 7 (COX-2: 259.2 +/- 190.4 / PGE2: 587.1 +/- 409.7). LLLT decreased Achilles tendon's inflammatory process. (AU)

• FAPESP's process: 11/00979-1 - Low level laser influence in the modulation of inflammation and tissue repair of rat Achilles tendon after partial injury
• Grantee: Helio Plapler
• Support Opportunities: Regular Research Grants