Androgen therapy reverses injuries caused by ethanol consumption in the prostate: Testosterone as a possible target to ethanol-related disorders

Autor(es)

Mendes, Leonardo O. ^[1] ; Scarano, Wellerson R. ^[2] ; Rochel-Maia, Sabrina S. ^[3] ; Fioruci-Fontaneli, Beatriz A. ^[1] ; Chuffa, Luiz G. A. ^[4] ; Anselmo-Franci, Janete A. ^[5] ; Martinez, Francisco E. ^[4]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Struct & Cell Biol Program, Campinas, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Morphol, BR-18618910 Botucatu, SP - Brazil

^[3] Inst Biosci Humanities & Exact Sci, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Anat, BR-18618910 Botucatu, SP - Brazil

^[5] Univ Sao Paulo, USP, Dept Morphol Stomatol & Physiol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 120, p. 22-30, JAN 1 2015.
• Citações Web of Science: 3

Resumo

Aims: Chronic ethanol consumption leads to reproductive damages, since it can act directly in the tissues or indirectly, causing a hormonal imbalance. Prostate is a hormone-dependent gland and, consequently, susceptible to ethanol. The potential of testosterone therapy in the ethanol-related disorders was investigated in the prostate microenvironment Main methods: UChB rats aged 90 days were divided into 2 experimental groups (n = 20): C: drinking water only and EtOH: drinking 10% (v/v) ethanol at >2 g/kg body weight! day + water. At 150 days old, 10 rats from each group received subcutaneous injections of testosterone cypionate (5 mg/kg body weight) diluted in corn oil every other day for 4 weeks, constituting T and EtOH+T, while the remaining animals received corn oil as vehicle. Animals were euthanized at 180 days old, by decapitation. Blood was collected to obtain hormone concentrations and ventral prostate was dissected and processed for light microscope and molecular analyses. Key findings: Ventral prostate weight, plasma testosterone and DHT and intraprostatic testosterone concentrations were increased after testosterone treatment Plasma estradiol level was reduced in the EtOH +T. Inflammatory foci, metaplasia and epithelial atrophy were constantly found in the prostate of EtOH and were not observed after hormonal therapy. No differences were found in the expression of AR, ER beta and DACH-1. Additionally, testosterone treatment down-regulated ER alpha and increased the e-cadherin and a-actinin immunoreactivities. Significance: Testosterone was able to reverse damages caused by ethanol consumption in the prostate microenvironment and becomes a possible target to be investigated to ethanol-related disorders. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/05936-9 - Terapia de reposição hormonal na próstata de ratos UChB: expressão de proteínas inflamatórias e de receptores hormonais
• Beneficiário: Francisco Eduardo Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/00917-9 - Terapia de reposição hormonal no lobo ventral da próstata de ratos UChB: efeitos sobre os receptores estrogênicos (er alfa e er beta) e na via de sinalização do TGF-beta 1
• Beneficiário: Leonardo de Oliveira Mendes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 09/54606-1 - Caracterizacao do processo inflamatorio e influencia da terapia de reposicao hormonal no lobo ventral da prostata de ratos bebedores voluntarios de etanol a 1o%(uchb)
• Beneficiário: Leonardo de Oliveira Mendes
• Modalidade de apoio: Bolsas no Brasil - Doutorado