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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pipa carvalhoi skin secretion profiling: Absence of peptides and identification of kynurenic acid as the major constitutive component

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Autor(es):
Ceolin Mariano, Douglas Oscar [1] ; Yamaguchi, Lydia Fumiko [2] ; Jared, Carlos [3] ; Antoniazzi, Marta Maria [3] ; Sciani, Juliana Mozer [1] ; Kato, Massuo Jorge [2] ; Pimenta, Daniel Carvalho [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Bioquim & Biofis, BR-05503900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo - Brazil
[3] Inst Butantan, Lab Biol Celular, BR-05503900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 167, p. 1-6, JAN 2015.
Citações Web of Science: 7
Resumo

The presence of peptides has been identified in all African pipid genera; nevertheless, little is known about skin secretion of South American frog genus Pipa. Skin secretion from captive and wild Pipa carvalhoi were obtained in the presence or absence of norepinephrine stimulation. The <10 kDa fraction was analyzed by liquid chromatography and mass spectrometry, searching for peptides. Chromatographic profiles show the presence of a major component in this secretion, regardless of the stimulation method (norepinephrine or mechanical stimulation) and the origin of the animal (captivity or wild), as well as in the absence of any stimulus. The general mass distribution profile in P. carvalhoi skin secretion shows numerous components below 800 Da. Moreover, no peptide could be identified, regardless of the chromatographic approach. The major component was purified and identified as kynurenic acid, an L-tryptophan derivative. P. carvalhoi does not secrete peptides as toxins in its skin. In addition, we here report that kynurenic acid is the main component of P. carvalhoi skin secretion. Although no biological activity was associated with kynurenic acid, we propose that this molecule is a pheromone that signals the presence of a co-specific in the shady environment in which this animal lives. In this study we demonstrate the absence of peptidic toxins in the skin secretion of P. carvalhoi, a break of paradigm in the pipid family. (C) 2014 Elsevier Inc All rights reserved. (AU)

Processo FAPESP: 12/02510-3 - ANÁLISE DO CONTEÚDO DA SECREÇÃO CUTÂNEA DO ANURO Pipa carvalhoi A PROCURA DE MOLÉCULAS COM POSSÍVEIS ATIVIDADES BIOLÓGICAS RELEVANTES
Beneficiário:Douglas Oscar Ceolin Mariano
Linha de fomento: Bolsas no Brasil - Mestrado