| Texto completo | |
| Autor(es): |
Boturao-Neto, Edmir
[1]
;
Yamamoto, Mihoko
[1]
;
Chiba, Akemi Kuroda
[1]
;
Sugano Kimura, Elisa Yuriko
[1]
;
Valgueiro Costa de Oliveira, Maria do Carmo
[2]
;
do Monte Barretto, Claudia Lumack
[2]
;
Alves Nunes, Mercia Maria
[2]
;
Lopes Albuquerque, Sergio Roberto
[3]
;
de Deus Santos, Marcos Daniel
[4]
;
Bordin, Jose Orlando
[1]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Hematol & Transfus Med Dept, BR-04023092 Sao Paulo, SP - Brazil
[2] Fundacao HEMOPE, Hemotherapy Dept, Recife, PE - Brazil
[3] Fundacao Hosp Hematol & Hemoterapia Estado Amazon, Immunohematol Dept, Manaus, AM - Brazil
[4] Univ Fed Espirito Santo, Dept Internal Med, Vila Velha, ES - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TRANSFUSION MEDICINE AND HEMOTHERAPY; v. 42, n. 1, p. 52-58, 2015. |
| Citações Web of Science: | 2 |
| Resumo | |
Background: KELnull (K-0) persons can produce clinically significant anti-KEL5 antibody after transfusion and/or pregnancy, requiring K-0 blood transfusion when indicated. 37 K-0 alleles have been reported in studies over different populations, but none in Amerindian-Caucasian descendants from South America. The aim of this study was to identify the molecular basis of K-0 phenotype in Brazilians. Methods: We investigated three K-0 samples from different Brazilian blood banks (Recife, Manaus, and Vila Velha) in women with anti-KEL5. KEL antigen typing was performed by serologic techniques, and the K-0 status was confirmed by flow cytometry. PCR-RFLP and DNA sequencing of the KEL coding and exon-intron regions were also performed. Results: RBCs of the 3 patients were phenotyped as KEL:-1,-2,-3,-4,-7. The 3 patients had the same KEL{*}02/02 genotype and were negative for KEL{*}02.03 and KEL{*}02.06 alleles. The Recife K-0 patient was homozygous for IVS16 + 1g>a mutation (KEL{*}02N.31 allele). The flow cytometry with anti-KEL1, anti-KEL2, anti-KEL3, anti-KEL4, and anti-CD238 confirmed the K-0 phenotype. In addition, we found the c.1042C>T mutation (KEL{*}02N.04 allele) in both the Manaus K-0 and the Vila Velha K-0 patients. Conclusion: This report represents the first study of K-0 molecular basis performed in Amerindian-Caucasian descendants from South America. (AU) | |
| Processo FAPESP: | 05/55237-9 - Aspectos clínicos e moleculares de antígenos e anticorpos de células sanguíneas |
| Beneficiário: | Jose Orlando Bordin |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |