Transdentinal Cell Photobiomodulation Using Different Wavelengths

Autor(es)

Turrioni, A. P. S. ^[1] ; Basso, F. G. ^[1] ; Alonso, J. R. L. ^[1] ; de Oliveira, C. F. ^[2] ; Hebling, J. ^[1] ; Bagnato, V. S. ^[3] ; de Souza Costa, C. A. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Araraquara Sch Dent, Dept Pediat Dent & Orthodont, Araraquara, SP - Brazil

^[2] Univ Ribeirao Preto, UNAERP, Dept Morphol, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil

^[4] Univ Estadual Paulista, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Operative Dentistry; v. 40, n. 1, p. 102-111, JAN-FEB 2015.
• Citações Web of Science: 5

Resumo

Objective: The aim of this study was to investigate the effects of transdentinal irradiation with different light-emitting diode (LED) parameters on odontoblast-like cells (MDPC-23). Methods and Materials: Human dentin discs (0.2 mm thick) were obtained, and cells were seeded on their pulp surfaces with complete culture medium (Dulbecco modified Eagle medium). Discs were irradiated from the occlusal surfaces with LED at different wavelengths (450, 630, and 840 nm) and energy densities (0, 4, and 25 J/cm(2)). Cell viability (methyltetrazolium assay), alkaline phosphatase activity (ALP), total protein synthesis (TP), and cell morphology (scanning electron microscopy) were evaluated. Gene expression of collagen type I (Col-I) was analyzed by quantitative polymerase chain reaction (PCR). Data were analyzed by the Mann-Whitney test with a 5% significance level. Results: Higher cell viability (21.8%) occurred when the cells were irradiated with 630 nm LED at 25 J/cm(2). Concerning TP, no statistically significant difference was observed between irradiated and control groups. A significant increase in ALP activity was observed for all tested LED parameters, except for 450 nm at 4 J/cm(2). Quantitative PCR showed a higher expression of Col-I by the cells subjected to infrared LED irradiation at 4 J/cm(2). More attached cells were observed on dentin discs subjected to irradiation at 25 J/cm(2) than at 4 J/cm(2). Conclusion: The infrared LED irradiation at an energy density of 4 J/cm(2) and red LED at an energy density of 25 J/cm(2) were the most effective parameters for transdentinal photobiomodulation of cultured odontoblast-like cells. (AU)

• Processo FAPESP: 09/03615-0 - Efeito transdentinário de diferentes tipos de LEDs sobre cultura de células odontoblásticas.
• Beneficiário: Ana Paula Silveira Turrioni
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/13895-0 - Efeito direto e transdentinário do LED infravermelho sobre células-tronco da polpa dentária humana in vitro e sobre o complexo dentino-pulpar in vivo
• Beneficiário: Ana Paula Silveira Turrioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado