Development and practical application of accelerated solvent extraction for the isolation of cocaine/crack biomarkers in meconium samples

Autor(es)

Mantovani, Cinthia de Carvalho ^[1] ; Lima, Marcela Bittar ^[1] ; Rodrigues de Oliveira, Carolina Dizioli ^[1] ; Menck, Rafael de Almeida ^[1] ; de Albuquerque Diniz, Edna Maria ^[2] ; Yonamine, Mauricio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Univ Hosp, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 957, p. 14-23, APR 15 2014.
• Citações Web of Science: 12

Resumo

A method using accelerated solvent extraction (ASE) for the isolation of cocaine/crack biomarkers in meconium samples, followed by solid phase extraction (SPE) and the simultaneous quantification by gas chromatography-mass spectrometry (GC-MS) was developed and validated. Initially, meconium samples were submitted to an ASE procedure, which was followed by SPE with Bond Elut Certify I cartridges. The analytes were derivatizated with PFP/PFPA and analyzed by GC-MS. The limits of detection CLOD) were between 11 and 17 ng/g for all analytes. The limits of quantification (LOQ) were 30 ng/g for anhydroecgonine methyl ester, and 20 ng/g for cocaine, benzoylecgonine, ecgonine methyl ester and cocaethylene. Linearity ranged from the LOQ to 1500 ng/g for all analytes, with a coefficients of determination greater than 0.991, except for m-hydroxybenzoylecgonine, which was only qualitatively detected. Precision and accuracy were evaluated at three concentration levels. For all analytes, inter-assay precision ranged from 3.2 to 18.1%. and intra-assay precision did not exceed 12.7%. The accuracy results were between 84.5 and 114.2% and the average recovery ranged from 17 to 84%. The method was applied to 342 meconium samples randomly collected in the University Hospital University of Sao Paulo (HU-USP), Brazil. Cocaine biomarkers were detected in 19 samples, which represent 5.6% of exposure prevalence. Significantly lower birth weight, length and head circumference were found for the exposed newborns compared with the non-exposed group. This is the first report in which ASE was used as a sample preparation technique to extract cocaine biomarkers from a complex biological matrix such as meconium samples. The advantages of the developed method are the smaller demand for organic solvents and the minor sample handling, which allows a faster and accurate procedure, appropriate to confirm fetal exposure to cocaine/crack. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/15483-4 - Uso da extração acelerada por solvente (ASE) para determinação cromatográfica de analitos de cocaína e tetraidrocanabinol em amostras de mecônio
• Beneficiário: Mauricio Yonamine
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/14108-2 - Uso da extração acelerada por solvente (ASE) para determinação cromatográfica de analitos de cocaína e tetraidrocanabinol em amostras de mecônio
• Beneficiário: Cinthia de Carvalho Mantovani
• Modalidade de apoio: Bolsas no Brasil - Mestrado