Alveolar bone dynamics in osteoporotic rats treated with raloxifene or alendronate: confocal microscopy analysis

Autor(es)

Ramalho-Ferreira, Gabriel ^{[1, 2]} ; Faverani, Leonardo Perez ^{[1, 2]} ; Grossi-Oliveira, Gustavo Augusto ^{[1, 2]} ; Okamoto, Tetuo ^{[1, 2]} ; Okamoto, Roberta ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Surg, BR-16015050 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, UNESP, Aracatuba Dent Sch, Integrated Clin, BR-16015050 Sao Paulo - Brazil

^[3] Univ Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Basic Sci, BR-16015050 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Optics; v. 20, n. 3 MAR 2015.
• Citações Web of Science: 5

Resumo

In this study, the characteristics of the alveolar bone of rats with induced osteoporosis were examined. Thirty-two rats were divided into four groups according to the induction of osteoporosis and drugs administered: OG, osteoporotic rats without treatment (negative control); SG, rats which underwent sham surgery ovariectomy (SHAM); alendronate (AG), osteoporotic rats treated with alendronate; and RG, osteoporotic rats treated with raloxifene (RG). On the 8th day after ovariectomy and SHAM surgeries, drug therapy was started with AG or RG. On the 52nd day, 20 mg/kg calcein was administered to all of the rats, and on the 80th day, 20 mg/kg alizarin red was administered. Euthanasia was performed on the 98th day. The bone area marked by fluorochromes was calculated and data were subjected to two-way ANOVA test and Tukey's post-hoc test (p < 0.05). The comparison of the induced osteoporosis groups showed no statistically significant differences in bone turnover only between RG and SG (p = 0.074) and AG and OG (p = 0.138). All other comparisons showed significant differences (p < 0.001). The largest bone turnover was observed in RG and SG groups. RG was the medication that improved the dynamics of the alveolar bone of rats with induced osteoporosis, resembling that of healthy rats. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU)

• Processo FAPESP: 10/13971-6 - Estudo in vitro das alterações na superfície do titânio comercialmente puro e da liga de titânio Ti -6Al-4V submetida a diferentes tratamentos
• Beneficiário: Leonardo Perez Faverani
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/15912-2 - Avaliação do reparo ósseo na interface osso / implante em ratas com osteoporose induzida tratadas com raloxifeno ou alendronato: análise histométrica, imunoistoquímica, por epifluorescência e biomecânica
• Beneficiário: Roberta Okamoto
• Modalidade de apoio: Auxílio à Pesquisa - Regular