Ferric chloride flocculation plus carbon adsorption allows to reuse spent culture medium of Arthrospira platensis

Autor(es)

Morocho-Jacome, Ana Lucia ^[1] ; Mascioli, Guilherme Favaro ^[1] ; Sato, Sunao ^[1] ; Monteiro de Carvalho, Joao Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENGINEERING IN LIFE SCIENCES; v. 15, n. 2, p. 208-219, MAR 2015.
• Citações Web of Science: 4

Resumo

Arthrospira platensis cultivation produces a saline spent medium that must be treated to allow its reuse, thus saving water and avoiding environmental pollution. This study evaluates the association of flocculation followed by adsorption to treat the spent medium by applying different concentrations of granular activated carbon (GAC) and ferric chloride (F), and using different residence times (T). The simultaneous optimization of the independent variables GAC, F, and T was performed using both a 2(3) central composite design and a response surface methodology. The cells cultivated in the medium obtained after the optimal conditions of treatment (GAC= 54.2 g L-1, F = 10.0 mg L-1, and T = 30.8 min) provided the highest maximum cell concentration, X-m = 3140 +/- 77 mg L-1 in 0.5 L Erlenmeyer flasks with the highest protein biomass content (44.9%). The treated medium in such conditions was also used in a 3.5 L tubular photobioreactor (PBR), reaching X-m = 4033 +/- 110 mg L-1 and biomass with high contents of both protein (47.3 +/- 2.6%) and chlorophyll (9.7 +/- 0.3 mg g dry cell(-1)). Therefore, this study can contribute to diminishing costs of A. platensis production by reusing its culture medium and improving its biomass quality in PBRs. (AU)

• Processo FAPESP: 10/52073-3 - Estudo do reaproveitamento de meio no cultivo de Arthrospira (Spirulina) platensis
• Beneficiário: Ana Lucia Morocho Jacome
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/52028-0 - Reaproveitamento de meio de cultivo de Arthrospira platensis utilizando tratamentos físico-químicos
• Beneficiário: João Carlos Monteiro de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular