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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pluronic F-127 hydrogel as a promising scaffold for encapsulation of dental-derived mesenchymal stem cells

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Autor(es):
Diniz, Ivana M. A. [1, 2] ; Chen, Chider [2] ; Xu, Xingtian [2] ; Ansari, Sahar [2, 3] ; Zadeh, Homayoun H. [3] ; Marques, Marcia M. [1] ; Shi, Songtao [2] ; Moshaverinia, Alireza [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo - Brazil
[2] Univ So Calif, Ostrow Sch Dent USC, Ctr Craniofacial Mol Biol, Los Angeles, CA 90033 - USA
[3] Univ So Calif, Ostrow Sch Dent USC, Lab Immunoregulat & Tissue Engn LITE, Los Angeles, CA 90033 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 26, n. 3 MAR 2015.
Citações Web of Science: 31
Resumo

Dental-derived mesenchymal stem cells (MSCs) provide an advantageous therapeutic option for tissue engineering due to their high accessibility and bioavailability. However, delivering MSCs to defect sites while maintaining a high MSC survival rate is still a critical challenge in MSC-mediated tissue regeneration. Here, we tested the osteogenic and adipogenic differentiation capacity of dental pulp stem cells (DPSCs) in a thermoreversible Pluronic F127 hydrogel scaffold encapsulation system in vitro. DPSCs were encapsulated in Pluronic (R) F-127 hydrogel and stem cell viability, proliferation and differentiation into adipogenic and osteogenic tissues were evaluated. The degradation profile and swelling kinetics of the hydrogel were also analyzed. Our results confirmed that Pluronic F-127 is a promising and nontoxic scaffold for encapsulation of DPSCs as well as control human bone marrow MSCs (hBMMSCs), yielding high stem cell viability and proliferation. Moreover, after 2 weeks of differentiation in vitro, DPSCs as well as hBMMSCs exhibited high levels of mRNA expression for osteogenic and adipogenic gene markers via PCR analysis. Our histochemical staining further confirmed the ability of Pluronic F-127 to direct the differentiation of these stem cells into osteogenic and adipogenic tissues. Furthermore, our results revealed that Pluronic F-127 has a dense tubular and reticular network morphology, which contributes to its high permeability and solubility, consistent with its high degradability in the tested conditions. Altogether, our findings demonstrate that Pluronic F-127 is a promising scaffold for encapsulation of DPSCs and can be considered for cell delivery purposes in tissue engineering. (AU)

Processo FAPESP: 13/17268-6 - Efeito da fototerapia a laser no transplante de hydrogel com incorporação de BMP4 e céluas-tronco para a regeneração do complexo dentino-pulpar in vivo
Beneficiário:Ivana Marcia Alves Diniz
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado