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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Pluronic F-127 hydrogel as a promising scaffold for encapsulation of dental-derived mesenchymal stem cells

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Author(s):
Diniz, Ivana M. A. [1, 2] ; Chen, Chider [2] ; Xu, Xingtian [2] ; Ansari, Sahar [2, 3] ; Zadeh, Homayoun H. [3] ; Marques, Marcia M. [1] ; Shi, Songtao [2] ; Moshaverinia, Alireza [2]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo - Brazil
[2] Univ So Calif, Ostrow Sch Dent USC, Ctr Craniofacial Mol Biol, Los Angeles, CA 90033 - USA
[3] Univ So Calif, Ostrow Sch Dent USC, Lab Immunoregulat & Tissue Engn LITE, Los Angeles, CA 90033 - USA
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 26, n. 3 MAR 2015.
Web of Science Citations: 31
Abstract

Dental-derived mesenchymal stem cells (MSCs) provide an advantageous therapeutic option for tissue engineering due to their high accessibility and bioavailability. However, delivering MSCs to defect sites while maintaining a high MSC survival rate is still a critical challenge in MSC-mediated tissue regeneration. Here, we tested the osteogenic and adipogenic differentiation capacity of dental pulp stem cells (DPSCs) in a thermoreversible Pluronic F127 hydrogel scaffold encapsulation system in vitro. DPSCs were encapsulated in Pluronic (R) F-127 hydrogel and stem cell viability, proliferation and differentiation into adipogenic and osteogenic tissues were evaluated. The degradation profile and swelling kinetics of the hydrogel were also analyzed. Our results confirmed that Pluronic F-127 is a promising and nontoxic scaffold for encapsulation of DPSCs as well as control human bone marrow MSCs (hBMMSCs), yielding high stem cell viability and proliferation. Moreover, after 2 weeks of differentiation in vitro, DPSCs as well as hBMMSCs exhibited high levels of mRNA expression for osteogenic and adipogenic gene markers via PCR analysis. Our histochemical staining further confirmed the ability of Pluronic F-127 to direct the differentiation of these stem cells into osteogenic and adipogenic tissues. Furthermore, our results revealed that Pluronic F-127 has a dense tubular and reticular network morphology, which contributes to its high permeability and solubility, consistent with its high degradability in the tested conditions. Altogether, our findings demonstrate that Pluronic F-127 is a promising scaffold for encapsulation of DPSCs and can be considered for cell delivery purposes in tissue engineering. (AU)

FAPESP's process: 13/17268-6 - Effect of laser phototherapy on the transplantation of a BMP4-loaded hydrogel plus stem cells for dentin-pulp complex regeneration in vivo
Grantee:Ivana Marcia Alves Diniz
Support Opportunities: Scholarships abroad - Research Internship - Doctorate