Biological and molecular characterization of a Trypanosoma cruzi isolate obtained from Panstrongylus megistus captured in Sao Paulo State, Brazil

Autor(es)

Martins, Luciamre P. A. ; Castanho, Roberto E. P. ; Therezo, Altino L. S. ^[1] ; Ribeiro, Aline R. ^[2] ; Lima, Luciana ^[3] ; Teixeira, Marta M. G. ^[3] ; Speranca, Marcia A. ^[4] ; Rodrigues, Vera L. C. ; da Rosa, Joao A. ^[5]

Afiliação do(s) autor(es)

^[1] Fac Med Marilia, Disciplina Anat Patol, Marilia - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Biol Anim, BR-13081970 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, BR-05508 Sao Paulo - Brazil

^[4] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre - Brazil

^[5] Unesp Araraquara, Disciplina Parasitol, Fac Ciencias Farmaceut, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA PARASITOLOGICA; v. 60, n. 1, p. 65-74, MAR 2015.
• Citações Web of Science: 0

Resumo

An isolate of Trypanosoma cruzi obtained from P. megistus captured in the peridomicile area of a home in Santo Antonio do Jardim city in the State of Sao Paulo, denominated T. cruzi Mogi, was characterized biologically and molecularly. The RFLP analysis of the D7 divergent domain in the 24S alpha rDNA and of the mini-exon positioned the T. cruzi isolate within the TcI group. Phylogenetic analysis performed with the trypanosomatid barcode confirmed that the isolate belongs to the TcI group, with high homology to the 3014 c1 T. cruzi strain. The biological characterization of the isolate in rats showed a prepatent period of about 8 days, low parasitemia and tropism for cardiac, skeletal and colonic muscles. In Swiss mice the T. cruzi Mogi isolate showed a prepatent period of about 22 days, intermittent parasitemia in some animals, and tropism for cardiac and colonic muscles. Despite the inherent difficulty of identifying correlations amongst the molecular and biological characteristics of different T. cruzi groups, the tropism for colonic muscle demonstrated by T. cruzi Mogi represented a peculiarity of this isolate within the TcI group. (AU)

• Processo FAPESP: 09/15824-3 - Isolamento e caracterização de cepas de Trypanosoma cruzi a partir de triatomíneos silvestres capturados na região de Marília
• Beneficiário: Luciamare Perinetti Alves Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular