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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biological and molecular characterization of a Trypanosoma cruzi isolate obtained from Panstrongylus megistus captured in Sao Paulo State, Brazil

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Author(s):
Martins, Luciamre P. A. ; Castanho, Roberto E. P. ; Therezo, Altino L. S. [1] ; Ribeiro, Aline R. [2] ; Lima, Luciana [3] ; Teixeira, Marta M. G. [3] ; Speranca, Marcia A. [4] ; Rodrigues, Vera L. C. ; da Rosa, Joao A. [5]
Total Authors: 9
Affiliation:
[1] Fac Med Marilia, Disciplina Anat Patol, Marilia - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Biol Anim, BR-13081970 Campinas, SP - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, BR-05508 Sao Paulo - Brazil
[4] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre - Brazil
[5] Unesp Araraquara, Disciplina Parasitol, Fac Ciencias Farmaceut, Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ACTA PARASITOLOGICA; v. 60, n. 1, p. 65-74, MAR 2015.
Web of Science Citations: 0
Abstract

An isolate of Trypanosoma cruzi obtained from P. megistus captured in the peridomicile area of a home in Santo Antonio do Jardim city in the State of Sao Paulo, denominated T. cruzi Mogi, was characterized biologically and molecularly. The RFLP analysis of the D7 divergent domain in the 24S alpha rDNA and of the mini-exon positioned the T. cruzi isolate within the TcI group. Phylogenetic analysis performed with the trypanosomatid barcode confirmed that the isolate belongs to the TcI group, with high homology to the 3014 c1 T. cruzi strain. The biological characterization of the isolate in rats showed a prepatent period of about 8 days, low parasitemia and tropism for cardiac, skeletal and colonic muscles. In Swiss mice the T. cruzi Mogi isolate showed a prepatent period of about 22 days, intermittent parasitemia in some animals, and tropism for cardiac and colonic muscles. Despite the inherent difficulty of identifying correlations amongst the molecular and biological characteristics of different T. cruzi groups, the tropism for colonic muscle demonstrated by T. cruzi Mogi represented a peculiarity of this isolate within the TcI group. (AU)

FAPESP's process: 09/15824-3 - Isolation and characterization of Trypanosoma cruzi strains from wild triatomines captured in the region of the city of Marília
Grantee:Luciamare Perinetti Alves Martins
Support Opportunities: Regular Research Grants