Autor(es)

Rossi, Guilherme Duarte ^{[1, 2]} ; Zucchi, Tiago Domingues ^{[3, 1]} ; Guidolin, Aline Sartori ^[1] ; Peruchi, Aline ^[1] ; Consoli, Fernando Luis ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, ESALQ, Dept Entomol & Acarol, Lab Interacoes Insetos, Piracicaba, SP - Brazil

^[2] UNESP, FCAV, Dept Fitossanidade, Jaboticabal, SP - Brazil

^[3] Embrapa Meio Ambiente, Lab Microbiol Ambiental, Jaguariuna, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANNALS OF MICROBIOLOGY; v. 65, n. 1, p. 565-574, MAR 2015.
• Citações Web of Science: 0

Resumo

Microbes have many mechanisms to exert their inhibitory activity against target pests. One such mechanism involves the production and secretion of hydrolytic enzymes, such as chitinases, which are produced naturally by plants in response to attack by insect herbivores and phytopathogens and have been sought as an additional factor to enhance pest management. Thus, our main aim was to screen the diverse actinomycete community associated with the integument of Acromyrmex subterraneus brunneus for a chitinase-producing strain and to characterize its chitinases. We identified isolate ENT-21-a Streptomyces sp.-as a chitinase-producer and our data indicate that this isolate produces a chitinolytic complex that contains a chitinase and a high-molecular-weight beta-N-acetylglucosaminidase (> 100 kDa) when cultured in Chitin-Czapek broth. The presence of chitinases in the genome of this isolate was checked by diagnostic PCR, and two chitinase genes belonging to family 18 group A and family 19 were verified. The chitinolytic activity of the crude extract was observed at pH values ranging from 3.8 to 11.0, with the highest chitinase activities recorded at pH 9.0 and 9.5, whereas optimum beta-N-acetylglucosaminidase activity was observed over a narrow pH range, between pH 4.7 and 5.1. We describe some biochemical and molecular properties of the chitinase and beta-N-acetylglucosaminidase produced by ENT-21, and discuss the potential for exploitation of these enzymes for pest control. (AU)

• Processo FAPESP: 07/58712-5 - Actinobactérias simbiontes de Acromyrmex sp.: fonte potencial de novas biomoléculas para o controle de pragas e doenças
• Beneficiário: Tiago Domingues Zucchi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/50877-0 - Diversidade, ecologia e potencial biotecnológico da bacteriofauna simbionte associada a insetos
• Beneficiário: Fernando Luis Cônsoli
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 11/14333-6 - Diversidade e taxonomia polifásica de actinobactérias isoladas de áreas de reflorestamento
• Beneficiário: Tiago Domingues Zucchi
• Modalidade de apoio: Bolsas no Brasil - Programa BIOTA - Jovens Pesquisadores
• Processo FAPESP: 10/13675-8 - Caracterização e aplicação bioindustrial da bacteriofauna intestinal de Armitermes euamignathus Silvestri, 1901 (Isoptera: Termitidae: Syntermitinae) e Heterotermes tenuis (Hagen, 1858) (Isoptera: Rhinotermitidae)
• Beneficiário: Aline Peruchi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/50021-1 - Caracterizacao funcional de proteinas do polidnavirus associado ao parasitoide cotesia flavipes (cameron)(hymenoptera,braconidae) na regulacao da resposta imunologica de diatraea saccharalis (fabr.)....
• Beneficiário: Guilherme Duarte Rossi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/59019-1 - Actinobacterias simbiontes de acromyrmex sp.: fonte potencial de novas biomoleculas para o controle de pragas e doencas.
• Beneficiário: Fernando Luis Cônsoli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/04287-0 - Toxoptera citricida (Kirkaldy) (Hemiptera: Aphidae): seus simbiontes e interações com simbiontes secundários
• Beneficiário: Aline Sartori Guidolin
• Modalidade de apoio: Bolsas no Brasil - Doutorado