Fetal kidney programming by severe food restriction: Effects on structure, hormonal receptor expression and urinary sodium excretion in rats

Autor(es)

Vaccari, Barbara ^[1] ; Mesquita, Flavia F. ^[2] ; Gontijo, Jose A. R. ^[2] ; Boer, Patricia A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Morphol, Botucatu, SP - Brazil

^[2] Univ Estadual Campinas, Sch Med, Dept Internal Med, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM; v. 16, n. 1, p. 33-46, MAR 2015.
• Citações Web of Science: 9

Resumo

Introduction: The present study investigates, in 23-day-old and adult male rats, the effect of severe food restriction in utero on blood pressure (BP), and its association with nephron structure and function changes, angiotensin II (AT1R/AT2R), glucocorticoid (GR) and mineralocorticoid (MR) receptor expression. Materials and methods: The daily food supply to pregnant rats was measured and one group (n=15) received normal quantity of food (NF) while the other received 50% of that (FR50%) (n=15). Kidneys were processed to AT1R, AT2R, MR, and GR immunolocalization and for western blotting analysis. The renal function was estimated by creatinine and lithium clearances in 12-week-old offspring. Results: By stereological analyses, FR50% offspring present a reduction of nephron numbers (35%) with unchanged renal volume. Expression of AT1R and AT2R was significantly decreased in FR50% while the expression of GR and MR increased in FR50%. We also verified a pronounced decrease in urinary sodium excretion accompanied by increased BP in 12-week-old FR50% offspring. Conclusion: The current data suggest that changes in renal function are conducive to excess sodium tubule reabsorption, and this might potentiate the programming of adult hypertension. It is plausible to arise in the current study an association between decreasing natriuresis, reciprocal changes in renal AngII and steroid receptors with the hypertension development found in FR50% compared with age-matched NF offspring. (AU)

• Processo FAPESP: 10/52696-0 - Estudo dos mecanismos envolvidos no desenvolvimento renal e na expressão hipocampo-hipotalamo-hipofisaria de receptores esteróides e dopaminérgicos em prole de ratas submetidas a restrição protéica gestacional
• Beneficiário: Jose Antonio Rocha Gontijo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/54362-4 - Biologia do desenvolvimento renal em modelo de restrição proteica durante a gestação em ratas
• Beneficiário: Patrícia Aline Boer
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 09/02719-7 - Desenvolvimento renal em um modelo de desnutrição e estresse gestacional
• Beneficiário: Bárbara Vaccari Cardoso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica