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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Exercise improves endothelial function: A local analysis of production of nitric oxide and reactive oxygen species

Texto completo
Autor(es):
Tanaka, Leonardo Yuji [1, 2] ; Grassmann Bechara, Luiz Roberto [1] ; dos Santos, Adriana Marques [1] ; Jordao, Camila Paixao [1] ; Oliveira de Sousa, Luis Gustavo [1] ; Bartholomeu, Teresa [1] ; Ventura, Laura Ines [2] ; Martins Laurindo, Francisco Rafael [2] ; Ramires, Paulo Rizzo [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Phys Educ & Sport, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Vasc Biol Lab, Inst Heart, BR-05403000 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 45, p. 7-14, FEB 15 2015.
Citações Web of Science: 21
Resumo

This study aimed at investigating the acute effects of aerobic exercise on endothelium-dependent vasomotor function of rat aorta, as well as mechanisms involved in endothelial nitric oxide (NO) bioactivity. Wistar rats were assigned to either a resting control (C, n = 21) or acutely exercised (E, n = 21) groups (60 min, 55-60% of maximum speed). After exercise, thoracic aorta was excised and cut into rings. Two rings were promptly applied to evaluate vasomotor function and the rest of aorta was used for additional measurements. Acute exercise significantly improved maximum ACh-induced relaxation (C, 91.6 +/- 1.2 vs. E, 102.4 +/- 1.7%, p < 0.001) and sensitivity to ACh (C, -7.3 +/- 0.06 vs. E, -7.3 +/- 0.02 log M, p < 0.01), and was accompanied by significantly increases on serine1177 eNOS phosphorylation, reflecting its enhanced activation. However, acute exercise also enhanced both superoxide and hydrogen peroxide production, as assayed by dihydroethidium oxidation, lucigenin chemiluminescence and Amplex Red assays. We also provided evidence for Nox2 NADPH oxidase (Nox) activation through gp91dstat-mediated inhibition of superoxide signals. Enhanced arterial relaxations associated with acute exercise were nearly-completely prevented by catalase, suggesting a role for paracrine hydrogen peroxide. Despite increased detectable oxidant generation, cellular oxidative stress was not evident, as suggested by unaltered GSH:GSSG ratio and lipid hydroperoxides. Collectively, these results demonstrate that one bout of moderate aerobic exercise improves endothelial function by increasing NO bioavailability, while superoxide and hydrogen peroxide are generated in a controlled fashion. (C) 2015 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 09/51654-5 - Dissulfeto isomerase proteica como via integrativa entre estresse oxidativo e resposta a proteínas mal-enoveladas na reparação a lesão vascular
Beneficiário:Leonardo Yuji Tanaka
Linha de fomento: Bolsas no Brasil - Doutorado