Hybrid Membranes of PLLA/Collagen for Bone Tissue Engineering: A Comparative Study of Scaffold Production Techniques for Optimal Mechanical Properties and Osteoinduction Ability

Autor(es)

Goncalves, Flavia ^[1] ; Bentini, Ricardo ^[1] ; Burrows, Mariana C. ^[1] ; Carreira, Ana C. O. ^{[1, 2]} ; Kossugue, Patricia M. ^[2] ; Sogayar, Mari C. ^{[1, 2]} ; Catalani, Luiz H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Nucleo Terapia Celular & Mol NUCEL, NETCEM, BR-05360130 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 8, n. 2, p. 408-423, 2015.
• Citações Web of Science: 16

Resumo

Synthetic and natural polymer association is a promising tool in tissue engineering. The aim of this study was to compare five methodologies for producing hybrid scaffolds for cell culture using poly-l-lactide (PLLA) and collagen: functionalization of PLLA electrospun by (1) dialkylamine and collagen immobilization with glutaraldehyde and by (2) hydrolysis and collagen immobilization with carbodiimide chemistry; (3) co-electrospinning of PLLA/chloroform and collagen/hexafluoropropanol (HFP) solutions; (4) co-electrospinning of PLLA/chloroform and collagen/acetic acid solutions and (5) electrospinning of a co-solution of PLLA and collagen using HFP. These materials were evaluated based on their morphology, mechanical properties, ability to induce cell proliferation and alkaline phosphatase activity upon submission of mesenchymal stem cells to basal or osteoblastic differentiation medium (ODM). Methods (1) and (2) resulted in a decrease in mechanical properties, whereas methods (3), (4) and (5) resulted in materials of higher tensile strength and osteogenic differentiation. Materials yielded by methods (2), (3) and (5) promoted osteoinduction even in the absence of ODM. The results indicate that the scaffold based on the PLLA/collagen blend exhibited optimal mechanical properties and the highest capacity for osteodifferentiation and was the best choice for collagen incorporation into PLLA in bone repair applications. (AU)

• Processo FAPESP: 10/17698-2 - Uso de matrizes poliméricas eletrofiadas e células tronco de polpa dentária e de adipócitos na engenharia dos tecidos periodontais
• Beneficiário: Flavia Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/21442-6 - Polímeros sintéticos e naturais aplicados à engenharia de tecido
• Beneficiário: Luiz Henrique Catalani
• Modalidade de apoio: Auxílio à Pesquisa - Temático