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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hybrid Membranes of PLLA/Collagen for Bone Tissue Engineering: A Comparative Study of Scaffold Production Techniques for Optimal Mechanical Properties and Osteoinduction Ability

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Goncalves, Flavia [1] ; Bentini, Ricardo [1] ; Burrows, Mariana C. [1] ; Carreira, Ana C. O. [1, 2] ; Kossugue, Patricia M. [2] ; Sogayar, Mari C. [1, 2] ; Catalani, Luiz H. [1]
Total Authors: 7
[1] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Nucleo Terapia Celular & Mol NUCEL, NETCEM, BR-05360130 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MATERIALS; v. 8, n. 2, p. 408-423, 2015.
Web of Science Citations: 16

Synthetic and natural polymer association is a promising tool in tissue engineering. The aim of this study was to compare five methodologies for producing hybrid scaffolds for cell culture using poly-l-lactide (PLLA) and collagen: functionalization of PLLA electrospun by (1) dialkylamine and collagen immobilization with glutaraldehyde and by (2) hydrolysis and collagen immobilization with carbodiimide chemistry; (3) co-electrospinning of PLLA/chloroform and collagen/hexafluoropropanol (HFP) solutions; (4) co-electrospinning of PLLA/chloroform and collagen/acetic acid solutions and (5) electrospinning of a co-solution of PLLA and collagen using HFP. These materials were evaluated based on their morphology, mechanical properties, ability to induce cell proliferation and alkaline phosphatase activity upon submission of mesenchymal stem cells to basal or osteoblastic differentiation medium (ODM). Methods (1) and (2) resulted in a decrease in mechanical properties, whereas methods (3), (4) and (5) resulted in materials of higher tensile strength and osteogenic differentiation. Materials yielded by methods (2), (3) and (5) promoted osteoinduction even in the absence of ODM. The results indicate that the scaffold based on the PLLA/collagen blend exhibited optimal mechanical properties and the highest capacity for osteodifferentiation and was the best choice for collagen incorporation into PLLA in bone repair applications. (AU)

FAPESP's process: 10/17698-2 - Use of electrospun polymer matrices and dental pulp and adipocytes stem cells in periodontal tissue engineering
Grantee:Flavia Gonçalves
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/21442-6 - Synthetic and natural polymers applied to tissue engineering
Grantee:Luiz Henrique Catalani
Support type: Research Projects - Thematic Grants