Hybrid Membranes of PLLA/Collagen for Bone Tissue Engineering: A Comparative Study of Scaffold Production Techniques for Optimal Mechanical Properties and Osteoinduction Ability

Author(s)

Goncalves, Flavia ^[1] ; Bentini, Ricardo ^[1] ; Burrows, Mariana C. ^[1] ; Carreira, Ana C. O. ^{[1, 2]} ; Kossugue, Patricia M. ^[2] ; Sogayar, Mari C. ^{[1, 2]} ; Catalani, Luiz H. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Nucleo Terapia Celular & Mol NUCEL, NETCEM, BR-05360130 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: MATERIALS; v. 8, n. 2, p. 408-423, 2015.
• Web of Science Citations: 16

Abstract

Synthetic and natural polymer association is a promising tool in tissue engineering. The aim of this study was to compare five methodologies for producing hybrid scaffolds for cell culture using poly-l-lactide (PLLA) and collagen: functionalization of PLLA electrospun by (1) dialkylamine and collagen immobilization with glutaraldehyde and by (2) hydrolysis and collagen immobilization with carbodiimide chemistry; (3) co-electrospinning of PLLA/chloroform and collagen/hexafluoropropanol (HFP) solutions; (4) co-electrospinning of PLLA/chloroform and collagen/acetic acid solutions and (5) electrospinning of a co-solution of PLLA and collagen using HFP. These materials were evaluated based on their morphology, mechanical properties, ability to induce cell proliferation and alkaline phosphatase activity upon submission of mesenchymal stem cells to basal or osteoblastic differentiation medium (ODM). Methods (1) and (2) resulted in a decrease in mechanical properties, whereas methods (3), (4) and (5) resulted in materials of higher tensile strength and osteogenic differentiation. Materials yielded by methods (2), (3) and (5) promoted osteoinduction even in the absence of ODM. The results indicate that the scaffold based on the PLLA/collagen blend exhibited optimal mechanical properties and the highest capacity for osteodifferentiation and was the best choice for collagen incorporation into PLLA in bone repair applications. (AU)

• FAPESP's process: 10/17698-2 - Use of electrospun polymer matrices and dental pulp and adipocytes stem cells in periodontal tissue engineering
• Grantee: Flavia Gonçalves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/21442-6 - Synthetic and natural polymers applied to tissue engineering
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants