Spatiotemporal distribution of different extracellular polymeric substances and filamentation mediate Xylella fastidiosa adhesion and biofilm formation

Autor(es)

Janissen, Richard ^[1] ; Murillo, Duber M. ^[1] ; Niza, Barbara ^[2] ; Sahoo, Prasana K. ^[1] ; Nobrega, Marcelo M. ^[3] ; Cesar, Carlos L. ^[4] ; Temperini, Marcia L. A. ^[3] ; Carvalho, Hernandes F. ^[5] ; de Souza, Alessandra A. ^[2] ; Cotta, Monica A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Dept Appl Phys, BR-13083859 Campinas, SP - Brazil

^[2] Agron Inst Campinas, Citrus Ctr APTA Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil

^[3] Univ Sao Paulo, Inst Chem, Fundamental Chem Dept, BR-05508000 Sao Paulo - Brazil

^[4] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Quantum Elect Dept, BR-13083859 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Inst Biol, Struct & Funct Biol Dept, BR-13083865 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 5, APR 20 2015.
• Citações Web of Science: 37

Resumo

Microorganism pathogenicity strongly relies on the generation of multicellular assemblies, called biofilms. Understanding their organization can unveil vulnerabilities leading to potential treatments; spatially and temporally-resolved comprehensive experimental characterization can provide new details of biofilm formation, and possibly new targets for disease control. Here, biofilm formation of economically important phytopathogen Xylella fastidiosa was analyzed at single-cell resolution using nanometer-resolution spectro-microscopy techniques, addressing the role of different types of extracellular polymeric substances (EPS) at each stage of the entire bacterial life cycle. Single cell adhesion is caused by unspecific electrostatic interactions through proteins at the cell polar region, where EPS accumulation is required for more firmly-attached, irreversibly adhered cells. Subsequently, bacteria form clusters, which are embedded in secreted loosely-bound EPS, and bridged by up to ten-fold elongated cells that form the biofilm framework. During biofilm maturation, soluble EPS forms a filamentous matrix that facilitates cell adhesion and provides mechanical support, while the biofilm keeps anchored by few cells. This floating architecture maximizes nutrient distribution while allowing detachment upon larger shear stresses; it thus complies with biological requirements of the bacteria life cycle. Using new approaches, our findings provide insights regarding different aspects of the adhesion process of X. fastidiosa and biofilm formation. (AU)

• Processo FAPESP: 10/18107-8 - Estudo das estruturas de nanofibras de PANI e de seus híbridos com nanoestruturas metálicas por espectroscopia Raman
• Beneficiário: Marcelo Medre Nobrega
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/50712-9 - Papel biologico do modulo toxina-antitoxina no mecanismo de morte celular programada em xylella fastidiosa.
• Beneficiário: Alessandra Alves de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51748-7 - Análise estrutural e química de biofilmes de Xylella fastidiosa
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático