Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spatiotemporal distribution of different extracellular polymeric substances and filamentation mediate Xylella fastidiosa adhesion and biofilm formation

Full text
Author(s):
Janissen, Richard [1] ; Murillo, Duber M. [1] ; Niza, Barbara [2] ; Sahoo, Prasana K. [1] ; Nobrega, Marcelo M. [3] ; Cesar, Carlos L. [4] ; Temperini, Marcia L. A. [3] ; Carvalho, Hernandes F. [5] ; de Souza, Alessandra A. [2] ; Cotta, Monica A. [1]
Total Authors: 10
Affiliation:
[1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Dept Appl Phys, BR-13083859 Campinas, SP - Brazil
[2] Agron Inst Campinas, Citrus Ctr APTA Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil
[3] Univ Sao Paulo, Inst Chem, Fundamental Chem Dept, BR-05508000 Sao Paulo - Brazil
[4] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Quantum Elect Dept, BR-13083859 Campinas, SP - Brazil
[5] Univ Estadual Campinas, Inst Biol, Struct & Funct Biol Dept, BR-13083865 Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 5, APR 20 2015.
Web of Science Citations: 37
Abstract

Microorganism pathogenicity strongly relies on the generation of multicellular assemblies, called biofilms. Understanding their organization can unveil vulnerabilities leading to potential treatments; spatially and temporally-resolved comprehensive experimental characterization can provide new details of biofilm formation, and possibly new targets for disease control. Here, biofilm formation of economically important phytopathogen Xylella fastidiosa was analyzed at single-cell resolution using nanometer-resolution spectro-microscopy techniques, addressing the role of different types of extracellular polymeric substances (EPS) at each stage of the entire bacterial life cycle. Single cell adhesion is caused by unspecific electrostatic interactions through proteins at the cell polar region, where EPS accumulation is required for more firmly-attached, irreversibly adhered cells. Subsequently, bacteria form clusters, which are embedded in secreted loosely-bound EPS, and bridged by up to ten-fold elongated cells that form the biofilm framework. During biofilm maturation, soluble EPS forms a filamentous matrix that facilitates cell adhesion and provides mechanical support, while the biofilm keeps anchored by few cells. This floating architecture maximizes nutrient distribution while allowing detachment upon larger shear stresses; it thus complies with biological requirements of the bacteria life cycle. Using new approaches, our findings provide insights regarding different aspects of the adhesion process of X. fastidiosa and biofilm formation. (AU)

FAPESP's process: 10/18107-8 - Study of the structures of PANI nanofibers and their hybrids with metallic nanostructures by Raman epectroscopy
Grantee:Marcelo Medre Nobrega
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/51748-7 - Chemical and structural analysis of Xylella fastidiosa biofilms
Grantee:Mônica Alonso Cotta
Support Opportunities: Regular Research Grants