Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods

Autor(es)

de Paula, Fabiana Martins ^{[1, 2]} ; Malta, Fernanda de Mello ^{[1, 3]} ; Marques, Priscilla Duarte ^[1] ; Sitta, Renata Barnabe ^[1] ; Rebello Pinho, Joao Renato ^{[1, 3]} ; Borges Gryschek, Ronaldo Cesar ^{[1, 2]} ; Chieffi, Pedro Paulo ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Fac Med, Inst Trop Med, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Molestias Infecciosas & Parasitarias, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Gastroenterol, Sao Paulo, SP - Brazil

^[4] Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 110, n. 2, p. 272-274, APR 2015.
• Citações Web of Science: 10

Resumo

This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas. (AU)

• Processo FAPESP: 10/51110-2 - Investigação de técnicas diagnósticas para identificação da infecção por S. stercoralis em pacientes com diferentes formas de imunodepressão
• Beneficiário: Pedro Paulo Chieffi
• Modalidade de apoio: Auxílio à Pesquisa - Regular