Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods

Author(s)

de Paula, Fabiana Martins ^{[1, 2]} ; Malta, Fernanda de Mello ^{[1, 3]} ; Marques, Priscilla Duarte ^[1] ; Sitta, Renata Barnabe ^[1] ; Rebello Pinho, Joao Renato ^{[1, 3]} ; Borges Gryschek, Ronaldo Cesar ^{[1, 2]} ; Chieffi, Pedro Paulo ^{[4, 1]}

Affiliation

^[1] Univ Sao Paulo, Hosp Clin, Fac Med, Inst Trop Med, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Molestias Infecciosas & Parasitarias, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Gastroenterol, Sao Paulo, SP - Brazil

^[4] Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Memórias do Instituto Oswaldo Cruz; v. 110, n. 2, p. 272-274, APR 2015.
• Web of Science Citations: 10

Abstract

This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas. (AU)

• FAPESP's process: 10/51110-2 - Investigation of diagnostic techniques for identification of infection S. stercoratis in patients with different forms of immunosuppression
• Grantee: Pedro Paulo Chieffi
• Support Opportunities: Regular Research Grants