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Evaluation of PCR technique efficiency with specific identification of the etiologic agent for canine visceral leishmaniasis in the city of Embu das Artes

Grant number: 11/04487-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): June 30, 2013
Field of knowledge:Biological Sciences - Parasitology - Protozoology of Parasites
Principal Investigator:Paulo Cesar Cotrim
Grantee:Thaynan Fernandes Cunha Martins
Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The classical diagnosis of canine visceral leishmaniasis (CVL) basically consists in the isolation of parasite in vitro, its identification by microscopic analysis and/or serological tests. It is possible to infer that these methods are difficult to be implemented, time-consuming and present low sensitivity and/or specificity and are incapable to differentiate the parasite species involved. With the development of molecular methods such as Polymerase Chain Reaction (PCR), the diagnosis of CVL became more accurate and easy to perform. The aim of this project is to evaluate the sensitivity and specificity of PCR in the diagnosis of CVL, using different tissues of dogs seropositive for leishmaniasis in the city of Embu das Artes - SP, and analyze the ability of this method to detecting the disease-causing agents involved in this infection . For that, three molecular targets were selected for amplification: 1) conserved region of minicircle kDNA, 2) region that encodes the glucose-6-phosphate dehydrogenase enzyme (G6PD), and 3) region of the gene encoding the heat shock protein hsp70. Standardizing the collection of biological samples, we intended to compare the results obtained in the PCR reactions with samples from different tissues, with the data obtained in the parasitological and serological tests, as well with the clinical data observed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA, BIANCA A.; MARTINS, THAYNAN F. C.; COSER, ELIZABETH M.; OLIVEIRA, VIVIANE DA L.; YAMASHIRO-KANASHIRO, EDITE H.; ROCHA, MUSSYA C.; PINTO, MARCELO M.; COTRIM, PAULO C.; COELHO, ADRIANO C.. Isolation, typing, and drug susceptibility of Leishmania (Leishmania) infantum isolates from dogs of the municipality of Embu das Artes, an endemic region for canine leishmaniasis in Brazil. Parasitology Research, v. 121, n. 9, p. 13-pg., . (11/04487-6, 18/03299-0, 20/01948-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARTINS, Thaynan Fernandes Cunha. Evaluation of PCR techniques for the identification and characterization of Visceral Leishmaniasis in different tissues of seropositive dogs. 2013. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT) São Paulo.

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