Investigation of diagnostic techniques for identification of infection S. stercoratis in patients with different forms of immunosuppression

• Grant number: 10/51110-2
• Support Opportunities: Regular Research Grants
• Field of knowledge: Biological Sciences - Parasitology - Helminthology of Parasites
• Principal Investigator: Pedro Paulo Chieffi
• Grantee: Pedro Paulo Chieffi
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Infection with S. stercoralis is potentially severe in immunosuppressed patients, especially those who use steroids. Stool traditional techniques have low sensitivity. Thus, the availability of sensitive and specific diagnostic techniques is desirable, especially in the context of immunodeficiencies, where,the identification and treatment of helminthiasis are mandatory. Therefore, it is proposed to implement and validate the techniques of culture agar plate, the indirect immunofluorescence test, the ELISA and the Western blot for the diagnosis of strongyloidiasis in patients with different forms of immunosuppression, in addition to the identification of antigens from filariform larvae of S. venezuelensis viable for the standardization of serological diagnosis. Finally we will promote the standardization of the extraction of DNA for molecular diagnosis of this helminthiasis using the PCR technique. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOLEDO, BEATRIZ; CORRAL, MARCELO A.; MEISEL, DIRCE MARY C. L.; GOTTARDI, MAIARA; ABDALA, EDSON; COSTA, SILVIA F.; PIERROTTI, LIGIA CAMERA; LESCANO, SUSANA A. Z.; GONCALVES, ELENICE M. N.; CASTILHO, VERA L. P.; et al. Screening of Strongyloides infection using an ELISA test in transplant candidates. Clinics, v. 74, 2019. (13/04236-9, 10/51110-2)

CORRAL, MARCELO ANDREETTA; DE PAULA, FABIANA MARTINS; MEISEL, DIRCE MARY C. L.; ABDALA, EDSON; COSTA, SILVIA FIGUEIREDO; PIERROTTI, LIGIA CAMERA; YAMASHIRO, JULIANA; DO NASCIMENTO GONCALVES, ELENICE M.; CASTILHO, VERA LUCIA P.; CHIEFFI, PEDRO PAULO; et al. IgG reactivity with 40-35 kDa soluble and membrane antigen of Strongyloides venezuelensis in immunocompromised patients. Acta Tropica, v. 190, p. 357-360, FEB 2019. (10/51110-2, 13/04236-9)

GOTTARDI, MAIARA; PAULA, FABIANA M.; CORRAL, MARCELO A.; MEISEL, DIRCE MARY C.; COSTA, SILVIA F.; ABDALA, EDSON; PIERROTTI, LIGIA C.; YAMASHIRO, JULIANA; CHIEFFI, PEDRO PAULO; GRYSCHEK, RONALDO CESAR B.. Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients. INFECTIOUS DISEASES, v. 47, n. 8, p. 550-554, AUG 2015. (10/51110-2)

SITTA, R. B.; MALTA, F. M.; PINHO, J. R.; CHIEFFI, P. P.; GRYSCHEK, R. C. B.; PAULA, F. M.. Conventional PCR for molecular diagnosis of human strongyloidiasis. Parasitology, v. 141, n. 5, p. 716-721, APR 2014. (10/51110-2)

PAULA, FABIANA MARTINS; SITTA, RENATA BARNABE; MALTA, FERNANDA MELLO; GOTTARDI, MAIARA; CORRAL, MARCELO ANDREETTA; BORGES GRYSCHEK, RONALDO CESAR; CHIEFFI, PEDRO PAULO. PARASITOLOGICAL AND MOLECULAR DIAGNOSIS IN EXPERIMENTAL Strongyloides venezuelensis INFECTION. Revista do Instituto de Medicina Tropical de São Paulo, v. 55, n. 2, p. 141-143, MAR-APR 2013. (10/51110-2)

PAULA, FABIANA M.; MALTA, FERNANDA M.; MARQUES, PRISCILLA D.; MELO, GESSICA B.; CORRAL, MARCELO A.; GOTTARDI, MAIARA; PINHO, JOAO R. R.; GONCALVES, ELENICE M. N.; CASTILHO, VERA L. P.; PIERROTTI, LIGIA C.; et al. Molecular diagnosis of Strongyloides stercoralis among transplant candidates. TRANSPLANT INFECTIOUS DISEASE, v. 20, n. 4, AUG 2018. (10/51110-2)

DE PAULA, FABIANA MARTINS; MALTA, FERNANDA MELLO; CORRAL, MARCELO ANDREETTA; MARQUES, PRISCILLA DUARTE; GOTTARDI, MAIARA; CORREIA LIMA MEISEL, DIRCE MARY; YAMASHIRO, JULIANA; REBELLO PINHO, JOAO RENATO; PAGLIUSI CASTILHO, VERA LUCIA; DO NASCIMENTO GONCALVES, ELENICE MESSIAS; et al. DIAGNOSIS OF Strongyloides stercoralis INFECTION IN IMMUNOCOMPROMISED PATIENTS BY SEROLOGICAL AND MOLECULAR METHODS. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, 2016. (10/51110-2, 13/04236-9)

SILVA, MARIA DO ROSARIO A.; MELO, GESSICA B.; MALTA, FERNANDA M.; ABDALA, EDSON; COSTA, SILVIA F.; PIERROTTI, LIGIA C.; GONCALVES, ELENICE M. N.; CASTILHO, VERA L. P.; CHIEFFI, PEDRO P.; GRYSCHEK, RONALDO C. B.; et al. Subtypes of Blastocystis sp. isolated in fecal samples from transplant candidates in Sao Paulo, Brazil. PARASITE EPIDEMIOLOGY AND CONTROL, v. 8, FEB 2020. (15/18213-6, 10/51110-2)

DE PAULA, FABIANA MARTINS; MALTA, FERNANDA DE MELLO; MARQUES, PRISCILLA DUARTE; SITTA, RENATA BARNABE; REBELLO PINHO, JOAO RENATO; BORGES GRYSCHEK, RONALDO CESAR; CHIEFFI, PEDRO PAULO. Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods. Memórias do Instituto Oswaldo Cruz, v. 110, n. 2, p. 272-274, APR 2015. (10/51110-2)

GRYSCHEK, RONALDO CESAR BORGES; CORRAL, MARCELO ANDREETTA; SITTA, RENATA BARNABE; GOTTARDI, MAIARA; PIERROTTI, LIGIA CAMERA; COSTA, SILVIA FIGUEIREDO; ABDALA, EDSON; CHIEFFI, PEDRO PAULO; DE PAULA, FABIANA MARTINS. Strongyloides infection screening in transplant candidates: What is the best strategy?. TRANSPLANT INFECTIOUS DISEASE, v. N/A, p. 8-pg., 2023-09-26. (10/51110-2, 13/04236-9)

PAULA, FABIANA MARTINS; SITTA, RENATA BARNABE; MALTA, FERNANDA MELLO; GOTTARDI, MAIARA; CORRAL, MARCELO ANDREETTA; BORGES GRYSCHEK, RONALDO CESAR; CHIEFFI, PEDRO PAULO. PARASITOLOGICAL AND MOLECULAR DIAGNOSIS IN EXPERIMENTAL Strongyloides venezuelensis INFECTION. Revista do Instituto de Medicina Tropical de São Paulo, v. 55, n. 2, p. 3-pg., 2013-03-01. (10/51110-2)