Novel nanocomposites based on a strawberry-like gold- coated magnetite (Fe@Au) for protein separation in multiple myeloma serum samples

Autor(es)

Araujo, Jose E. ^{[1, 2]} ; Lodeiro, Carlos ^{[1, 2]} ; Capelo, Jose L. ^{[1, 2]} ; Rodriguez-Gonzalez, Benito ^[3] ; dos Santos, Alcindo A. ^[4] ; Santos, Hugo M. ^{[1, 2]} ; Fernandez-Lodeiro, Javier ^{[4, 1, 2]}

Afiliação do(s) autor(es)

^[1] ProteoMass Sci Soc, P-2825182 Caparica - Portugal

^[2] Univ Nova Lisboa, Fac Sci & Technol, Dept Chem, BIOSCOPE Res Grp, UCIBIO REQUIMTE, P-2829516 Caparica - Portugal

^[3] Univ Vigo, Sci & Technol Res Assistance Ctr CACTI, Vigo 36310 - Spain

^[4] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NANO RESEARCH; v. 8, n. 4, p. 1189-1198, APR 2015.
• Citações Web of Science: 17

Resumo

A new process to produce magnetite partially coated with strawberry-like gold nanoparticles in aqueous media is reported. The fast response to magnetic fields and optical properties of gold nanoparticle-based colloidal systems are the two main advantages of this new Fe@Au nanomaterial. These advantages allow for the use of this new colloidal nanomaterial for various purposes in proteomics and biomedicine, as proteins can bind to the surface, and the surface can also be functionalized. As proof-of-concept, the new Fe@Au nanoparticles have been assessed in biomarker discovery as a tool for pre-concentration and separation of proteins from complex proteomes. To this end, sera from healthy people were compared with sera from patients diagnosed with multiple myeloma. The application of this new Fe@Au nanomaterial combined with mass spectrometry has allowed for the identification of 53 proteins, and it has also shown that the heat shock protein HSP75 and the plasma protease C1 inhibitor are potential biomarkers for diagnostics and control of multiple myeloma progression. (AU)

• Processo FAPESP: 08/55401-1 - Preparação e aplicação de compostos quirais contendo chalcogênios
• Beneficiário: João Valdir Comasseto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/11666-0 - Novos probes de fluorescência no infravermelho próximo baseados em calcogenetoes para espécies reativas de oxigênio (ERO)
• Beneficiário: Javier Fernández Lodeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/11613-8 - Reações de acoplamento-A3 na síntese de compostos nitrogenados bioativos
• Beneficiário: Alcindo Aparecido dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular