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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture

Texto completo
Autor(es):
de Souza Rocha-Frigoni, Nathalia Alves [1] ; da Silva Leao, Beatriz Caetano [1] ; Nogueira, Eriklis [1] ; Accorsi, Monica Ferreira [1] ; Mingoti, Gisele Zoccal [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Brazilian Agr Res Corp, EMBRAPA Pantanal, Corumba - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: ZYGOTE; v. 23, n. 2, p. 159-168, APR 2015.
Citações Web of Science: 7
Resumo

This study examined the effects of antioxidant supplementation and O-2 tension on embryo development, cryotolerance and intracellular reactive oxygen species (ROS) levels. The antioxidant supplementation consisted of 0.6 mM cysteine (CYST); 0.6 mM cysteine + 100 mu M cysteamine (C+C); 100 IU catalase (CAT) or 100 mu M beta-mercaptoethanol (beta-ME) for 3 or 7 days of in vitro culture (IVC). Two O-2 tensions (20% O-2 {[}5% CO2 in air] or 7% O-2, 5% CO2 and 88% N-2 {[}gaseous mixture]) were examined. After 7 days of antioxidant supplementation, the blastocyst frequencies were adversely affected (P<0.05) by CYST (11.2%) and C+C (1.44%), as well as by low O-2 tension (17.2% and 11.11% for 20% and 7% O-2, respectively) compared with the control (26.6%). The blastocyst re-expansion rates were not affected (P > 0.05) by the treatments (range, 66-100%). After 3 days of antioxidant supplementation, the blastocyst frequencies were not affected (P > 0.05) by any of the antioxidants (range, 43.6-48.5%), but they were reduced by low O-2 tension (P<0.05) (52.1% and 38.4% for 20% and 7% O-2, respectively). The intracellular ROS levels, demonstrated as arbitrary fluorescence units, were not affected (P > 0.05) by antioxidant treatment (range, 0.78 to 0.95) or by O-2 tension (0.86 and 0.88 for 20% and 7% O-2, respectively). The re-expansion rates were not affected (P > 0.05) by any of the treatments (range, 63.6-93.3%). In conclusion, intracellular antioxidant supplementation and low O-2 tension throughout the entire IVC period were deleterious to embryo development. However, antioxidant supplementation up to day 3 of IVC did not affect the blastocyst frequencies or intracellular ROS levels. (AU)

Processo FAPESP: 11/18257-2 - Criotolerância de embriões bovinos produzidos in vitro: efeitos da tensão de oxigênio e da suplementação do meio com antioxidantes em diferentes etapas do cultivo
Beneficiário:Gisele Zoccal Mingoti
Modalidade de apoio: Auxílio à Pesquisa - Regular