Determination of Tetracyclines in Bovine and Human Urine using a Graphite-Polyurethane Composite Electrode

Autor(es)

Fioramonti Calixto, Carolina Maria ^[1] ; Gomes Cavalheiro, Eder Tadeu ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytical Letters; v. 48, n. 9, p. 1454-1464, JUN 13 2015.
• Citações Web of Science: 10

Resumo

The performance of a bare graphite-polyurethane composite electrode (60 percent graphite, m/m) for the determination of tetracycline in human urine and oxytetracycline in human and bovine urine was evaluated. Using optimized differential pulse voltammetry, limits of detection as low as 2.6 micromoles per liter (tetracycline) and 1.7 micromoles per liter (oxytetracycline) were found in phosphate buffer at pH 2.5. The sample preparation included acidification and centrifugation to remove solid proteins followed by direct analysis. Recoveries between 96 and 100 percent were obtained for the determination of tetracycline and oxytetracycline in fortified urine. (AU)

• Processo FAPESP: 10/11080-7 - Preparação e Aplicação de Eletrodos Compósitos à Base de Grafite e Polímeros: Modificação com Sílicas Organofuncionalizadas e Polímeros Molecularmente Impressos
• Beneficiário: Éder Tadeu Gomes Cavalheiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/02590-1 - Utilização de eletrodos compósitos na análise voltamétrica de fármacos em amostras biológicas e/ou ambientais.
• Beneficiário: Carolina Maria Fioramonti Calixto
• Modalidade de apoio: Bolsas no Brasil - Doutorado