Determination of Tetracyclines in Bovine and Human Urine using a Graphite-Polyurethane Composite Electrode

Author(s)

Fioramonti Calixto, Carolina Maria ^[1] ; Gomes Cavalheiro, Eder Tadeu ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Analytical Letters; v. 48, n. 9, p. 1454-1464, JUN 13 2015.
• Web of Science Citations: 10

Abstract

The performance of a bare graphite-polyurethane composite electrode (60 percent graphite, m/m) for the determination of tetracycline in human urine and oxytetracycline in human and bovine urine was evaluated. Using optimized differential pulse voltammetry, limits of detection as low as 2.6 micromoles per liter (tetracycline) and 1.7 micromoles per liter (oxytetracycline) were found in phosphate buffer at pH 2.5. The sample preparation included acidification and centrifugation to remove solid proteins followed by direct analysis. Recoveries between 96 and 100 percent were obtained for the determination of tetracycline and oxytetracycline in fortified urine. (AU)

• FAPESP's process: 10/11080-7 - Preparation and application of grafite-polimer based composite electrodes : modification with organofunctionalized silicas and molecurlay imprinted polymers
• Grantee: Éder Tadeu Gomes Cavalheiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 08/02590-1 - Using composite electrodes in the voltammetric determination of pharmaceuticals in biological and/or environmental samples.
• Grantee: Carolina Maria Fioramonti Calixto
• Support Opportunities: Scholarships in Brazil - Doctorate