Photocytotoxicity of a cyanine dye with two chromophores toward melanoma and normal cells

Autor(es)

Murakami, L. S. ^[1] ; Ferreira, L. P. ^[1] ; Santos, J. S. ^[2] ; da Silva, R. S. ^{[3, 2]} ; Nomizo, A. ^[4] ; Kuz'min, V. A. ^[5] ; Borissevitch, I. E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin, BR-14040903 Ribeirao Preto, SP - Brazil

^[5] Russian Acad Sci, Emanuel Inst Biochem Phys, Moscow 117977 - Russia

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS; v. 1850, n. 6, p. 1150-1157, JUN 2015.
• Citações Web of Science: 10

Resumo

Background: Due to high optical absorption, triplet quantum yield and affinity to biological structures bichromophoric cyanine dyes (BCDs) can be considered promising sensitizers for application in photodynamic therapy (PDT). In this work, we report on the study of the BCD photocytotoxicity toward melanoma and normal cells in comparison with that of commercial photosensitizer Photogem (R). Methods: The cytotoxic and phototoxic effects were measured by standard tests of cell viability. The drug uptake was obtained by the flow cytometry and optical absorption techniques. The BCD intracellular distribution was obtained by the fluorescence image microscopy using specific organelle markers. Results: Both drugs demonstrated increased cytotoxicity under irradiation, while in darkness their cytotoxic effect at concentrations lower than 20 mu M after 24 h of incubation did not exceed 20%. For 5 h of incubation, BCD photocytotoxicity in relation to melanoma cells reached 100% already at concentrations below 5 mu M, while for normal cells the effect did not exceed 70% even for the 20 mu M concentration. It is shown that BCD penetrates into the cells and is located predominantly in perinuclear cytoplasmic structures. Conclusions: The BCD photosensitizing characteristics appear more adequate for application in PDT than that of the actually applied commercial photosensitizer Photogem (R). Higher light absorption by BCD in the near IR region and its preferential localization in mitochondria can explain its high photocytotoxicity. General significance: BCD can be considered as a new promising photosensitizer class for cancer PDT. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 01/07559-6 - Estudo da dinâmica do processo de agregação de porfirinas na presença de sistemas microorganizados
• Beneficiário: Lucimara Perpetua Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto