Aedes aegypti salivary gland extract ameliorates experimental inflammatory bowel disease

Autor(es)

Sales-Campos, Helioswilton ^[1] ; de Souza, Patricia Reis ^[1] ; Basso, Paulo Jose ^[2] ; Ramos, Anderson Daniel ^[3] ; Nardini, Viviani ^[1] ; Lazo Chica, Javier Emilio ^[4] ; Capurro, Margareth Lara ^[5] ; Sa-Nunes, Anderson ^[3] ; de Barros Cardoso, Cristina Ribeiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil

^[4] Univ Fed Triangulo Mineiro, Inst Ciencias Biol & Nat, Uberaba - Brazil

^[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Immunopharmacology; v. 26, n. 1, p. 13-22, MAY 2015.
• Citações Web of Science: 8

Resumo

Current therapies for inflammatory bowel disease (IBD) are not totally effective, resulting in persistent and recurrent disease for many patients. Mosquito saliva contains immunomodulatory molecules and therein could represent a novel therapy for IBD. Here, we demonstrated the therapeutic activity of salivary gland extract (SGE) of Aedes aegypti on dextran sulfate sodium (DSS)-induced colitis. For this purpose, C57BL/6 male mice were exposed to 3% DSS in drinking water and treated with SGE at early (days 3-5) or late (days 5-8) time points, followed by euthanasia on days 6 and 9, respectively, for sample collection. The results showed an improvement in clinical disease outcome and postmortem scores after SGE treatment accompanied by the systemic reduction in peripheral blood lymphocytes, with no impact on bone marrow and mesenteric lymph nodes cellularity or macrophages toxicity. Moreover, a local diminishment of IFN-gamma, TNE-alpha, IL-1 beta and IL-5 cytokines together with a reduction in the inflammatory area were observed in the colon of SGE-treated mice. Strikingly, early treatment with SGE led to mice protection from a late DSS re-challenging, as observed by decreased clinical and postmortem scores, besides reduced circulating lymphocytes, indicating that the mosquito saliva may present components able to prevent disease relapse. Indeed, high performance liquid chromatography (HPLC) experiments pointed to a major SGE pool fraction (F3) able to ameliorate disease signs. In conclusion, SGE and its components might represent a source of important immunomodulatory molecules with promising therapeutic activity for IBD. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/09892-6 - Imunoma funcional da saliva de Aedes aegypti
• Beneficiário: Anderson de Sá Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/20162-7 - Papel do eixo hipotálamo-pituitária adrenal e glicocorticóides exógenos na modulação da resposta imune na doença inflamatória intestinal
• Beneficiário: Cristina Ribeiro de Barros Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores