Comparison of four molecular methods to type Salmonella Enteritidis strains

Autor(es)

Campioni, Fabio ^[1] ; Pitondo-Silva, Andre ^[1] ; Bergamini, Alzira M. M. ^[2] ; Falcao, Juliana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Inst Adolfo Lutz Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APMIS; v. 123, n. 5, p. 422-426, MAY 2015.
• Citações Web of Science: 7

Resumo

This study compared the pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), multilocus variable-number of tanden-repeat analysis (MLVA), and multilocus sequence typing (MLST) methods for typing 188 Salmonella Enteritidis strains from different sources isolated over a 24-year period in Brazil. PFGE and ERIC-PCR were more efficient than MLVA for subtyping the strains. However, MLVA provided additional epidemiological information for those strains. In addition, MLST showed the Brazilian strains as belonging to the main clonal complex of S. Enteritidis, CC11, and provided the first report of two new STs in the S. enterica database but could not properly subtype the strains. Our results showed that the use of PFGE or ERIC-PCR together with MLVA is suitable to efficiently subtype S. Enteritidis strains and provide important epidemiological information. (AU)

• Processo FAPESP: 08/57478-1 - Tipagem molecular e analise da diversidade genetica de linhagens de salmonella enteritidis isoladas de humanos e de alimentos no estado de sao paulo, brasil.
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/09998-9 - Tipagem molecular e análise da diversidade genética de linhagens de Salmonella Enteritidis isoladas de humanos e de alimentos no Estado de São Paulo, Brasil.
• Beneficiário: Fábio Campioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado