Exposure to acids changes the proteomic of acquired dentine pellicle

Autor(es)

Delecrode, Taisa Ribas ^[1] ; Siqueira, Walter Luiz ^[2] ; Zaidan, Flavia Cardoso ^[1] ; Bellini, Melina Rodrigues ^[1] ; Leite, Aline Lima ^[1] ; Xiao, Yishi ^[2] ; Rios, Daniela ^[1] ; Magalhaes, Ana Carolina ^[1] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[2] Univ Western Ontario, Schulich Sch Med & Dent, London, ON N6A 5C1 - Canada

• Tipo de documento: Artigo Científico
• Fonte: Journal of Dentistry; v. 43, n. 5, p. 583-588, MAY 2015.
• Citações Web of Science: 10

Resumo

Objectives: For the first time, this study characterized the proteome of the acquired pellicle formed on human dentine. The changes in this proteome after exposure to lactic or citric acid were also evaluated. Methods: Volunteers (n = 9) wore a mandibular device containing 6 specimens of human root dentine. After the device remained in the volunteers' oral cavities for 10 min or 2 h to allow the formation of the acquired pellicle in situ, the specimens were immersed in citric acid (1%, pH 2.5) or lactic acid (0.1 M, pH 4.8) or deionized water for 20 s. In sequence, the pellicle was collected with an electrode filter paper soaked in 3% citric acid. This procedure was repeated for two additional days following a crossover protocol. After harvest, proteins were subjected to reverse phase liquid chromatography coupled to mass spectrometry (nLC-ESI-MS/MS). MS/MS data were processed and submitted to Proteome Discoverer software. Searches were done using SWISS-PROT and TrEMBL databases for human proteins. Results: In total, 223 distinct proteins were identified in the dentine acquired pellicle in each of the different conditions. Exposure to citric acid dramatically reduced the number of identified proteins. This did not occur for lactic acid. Acid-resistant proteins, such as mucins, were identified after pellicle was exposed to lactic or citric acid. Conclusions: These proteins could be related to protective effect of tooth homeostasis. Moreover, in the future, they could be candidates to the development of a supplemental therapy for the prevention and treatment of dental caries and dental erosion. Clinical significance: This study indicates some acid-resistant proteins that could be used in dental products to prevent dental caries and erosion. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/53852-9 - EMU: aquisição de um espectrômetro de massas híbrido com analisadores do tipo quádruplo e time-of-flight para identificação de peptídeos, proteínas e produtos do metabolismo
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 10/15216-0 - Determinação da composição da película adquirida formada sobre esmalte e dentina e da sua modificação após exposição ácida: estudo proteômico
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/11083-1 - Determinação da mudança na composição da película adquirida formada sobre esmalte e dentina após exposição ácida: estudo proteômico
• Beneficiário: Taísa Ribas Delecrode
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 11/03284-4 - Determinação da mudança na composição da película adquirida sobre esmalte e dentina após exposição ácida: estudo proteômico
• Beneficiário: Taísa Ribas Delecrode
• Modalidade de apoio: Bolsas no Brasil - Mestrado