Molecular detection and phylogenetic analysis of bovine astrovirus in Brazil

Autor(es)

Candido, Marcelo ^[1] ; Farias Alencar, Anna Luiza ^[1] ; Almeida-Queiroz, Sabrina R. ^[1] ; Buzinaro, Maria da Gloria ^[2] ; Munin, Flavia Simone ^[1] ; Seraphin de Godoy, Silvia Helena ^[1] ; Livonesi, Marcia Cristina ^[3] ; Fernandes, Andrezza Maria ^[1] ; Moro de Sousa, Ricardo Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FZEA USP, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Prevent Vet Med & Anim Reprod, BR-14884900 Sao Paulo - Brazil

^[3] Alfenas Fed Univ UNIFAL, Dept Clin Anal, BR-37130000 Alfenas, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 160, n. 6, p. 1519-1525, JUN 2015.
• Citações Web of Science: 8

Resumo

Bovine astrovirus (BoAstV) is associated with gastroenterical disorders such as diarrhea, particularly in neonates and immunocompromised animals. Its prevalence is > 60 % in the first five weeks of the animal's life. The aim of this study was to detect and perform a phylogenetic analysis of BoAstV in Brazilian cattle. A prevalence of 14.3 % of BoAstV in fecal samples from 272 head of cattle from different Brazilian states was detected, and 11 samples were analyzed by nucleotide sequencing. The majority of positive samples were obtained from diarrheic animals (p < 0.01). Phylogenetic analysis revealed that Brazilian samples were grouped in clades along with other BoAstV isolates. There was 74.3 %-96.5 % amino acid sequence similarity between the samples in this study and > 74.8 % when compared with reference samples for enteric BoAstV. Our results indicate, for the first time, the occurrence of BoAstV circulation in cattle from different regions of Brazil, prevalently in diarrheic calves. (AU)

• Processo FAPESP: 06/52060-3 - Epidemiologia das infecções por torovírus em bovinos no estado de São Paulo: do diagnóstico molecular por RT-PCR ao desenvolvimento e aplicação de ensaios imunoenzimático (das-elisa e i-elisa) [... ]
• Beneficiário: Ricardo Luiz Moro de Sousa
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/18441-0 - Detecção e caracterização moleculares de Astrovirus em bovinos das regiões nordeste do Estado de São Paulo e sudoeste do Estado de Minas Gerais
• Beneficiário: Marcelo Candido
• Modalidade de apoio: Bolsas no Brasil - Mestrado