Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis

Autor(es):Mostrar menos -

Machado, Carla Botelho ^[1] ; Citadini, Ana Paula ^[1] ; Goldbeck, Rosana ^[1] ; de Lima, Evandro Antonio ^[1] ; Figueiredo, Fernanda Lopes ^[1] ; da Silva, Tony Marcio ^[2] ; Hoffmam, Zaira Bruna ^[1] ; de Sousa, Amanda Silva ^[1] ; Squina, Fabio Marcio ^[1] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^[2] ; Ruller, Roberto ^[1] ; Ward, Richard John ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Butanta - Brazil

^[3] Univ Sao Paulo, Dept Chem, Fac Filosofia Ciencias & Letras Ribeirao Preto, Butanta - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biotechnology Letters; v. 37, n. 7, p. 1455-1462, JUL 2015.
• Citações Web of Science: 3

Resumo

The use of endo-arabinanase from Bacillus licheniformis (ABNase) for sugarcane saccharification has been evaluated by enzyme immobilization and commercial cocktail supplement with the immobilized heterologous protein. Biochemical characterization of the purified ABNase showed that the catalytic activity was strongly inhibited by 5 mM Cu2+, Zn2+ or Fe3+. The optimum pH and temperature for activity were 5.5-6.5 and 35-40 A degrees C, respectively. The enzyme stability increased 128-fold when immobilized with glyoxyl agarose, and the hydrolysis of pretreated sugar cane biomass increased by 15 % when a commercial enzyme cocktail was supplemented with immobilized ABNase. Pectin hydrolysis by recombinant ABNase plays a role in the effective application of enzymatic cocktails for biomass saccharification. (AU)

• Processo FAPESP: 10/18850-2 - Identificação, caracterização e engenharia de enzimas que degradam a parede celular das plantas
• Beneficiário: Richard John Ward
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/14454-3 - Bioprospecção de pectinases para suplementação de coquetéis enzimáticos visando à degradação da biomassa vegetal
• Beneficiário: Carla Botelho Machado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado