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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis

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Author(s):
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Machado, Carla Botelho [1] ; Citadini, Ana Paula [1] ; Goldbeck, Rosana [1] ; de Lima, Evandro Antonio [1] ; Figueiredo, Fernanda Lopes [1] ; da Silva, Tony Marcio [2] ; Hoffmam, Zaira Bruna [1] ; de Sousa, Amanda Silva [1] ; Squina, Fabio Marcio [1] ; Teixeira de Moraes Polizeli, Maria de Lourdes [2] ; Ruller, Roberto [1] ; Ward, Richard John [1, 3]
Total Authors: 12
Affiliation:
[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083970 Campinas, SP - Brazil
[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Butanta - Brazil
[3] Univ Sao Paulo, Dept Chem, Fac Filosofia Ciencias & Letras Ribeirao Preto, Butanta - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Biotechnology Letters; v. 37, n. 7, p. 1455-1462, JUL 2015.
Web of Science Citations: 3
Abstract

The use of endo-arabinanase from Bacillus licheniformis (ABNase) for sugarcane saccharification has been evaluated by enzyme immobilization and commercial cocktail supplement with the immobilized heterologous protein. Biochemical characterization of the purified ABNase showed that the catalytic activity was strongly inhibited by 5 mM Cu2+, Zn2+ or Fe3+. The optimum pH and temperature for activity were 5.5-6.5 and 35-40 A degrees C, respectively. The enzyme stability increased 128-fold when immobilized with glyoxyl agarose, and the hydrolysis of pretreated sugar cane biomass increased by 15 % when a commercial enzyme cocktail was supplemented with immobilized ABNase. Pectin hydrolysis by recombinant ABNase plays a role in the effective application of enzymatic cocktails for biomass saccharification. (AU)

FAPESP's process: 10/18850-2 - Identification, characterization and engineering of plant cell wall degrading enzymes
Grantee:Richard John Ward
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/14454-3 - Bioprospection of pectinase supplementation aimed enzyme cocktails to the degradation of plant biomass
Grantee:Carla Botelho Machado
Support Opportunities: Scholarships in Brazil - Post-Doctoral