Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia

Autor(es)

Iyomasa, Mamie Mizusaki ^[1] ; Fernandes, Fernanda Silva ^[1] ; Iyomasa, Daniela Mizusaki ^[1] ; Lara Pereira, Yamba Carla ^[2] ; Restrepo Fernandez, Rodrigo Alberto ^[3] ; Calzzani, Ricardo Alexandre ^[1] ; Nascimento, Glauce Crivelaro ^{[4, 1]} ; Andrade Leite-Panissi, Christie Ramos ^{[4, 1]} ; Mardegan Issa, Joao Paulo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Dent Fac, Dept Morphol Physiol & Basic Pathol, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Campinas, Sch Dent Piracicaba, Biol Dent Buco Grad Program, BR-13414903 Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Physiol, BR-14040900 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Sch Philosophy Sci & Literature Ribeirao Preto, Psychobiol Grad Program, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 6 JUN 8 2015.
• Citações Web of Science: 3

Resumo

Clinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evaluate the influence of acute stress on metabolic activity and oxidative stress of masseter muscles of rats subjected to occlusal modification through morphological and histochemical analyses. In this study, adult Wistar rats were divided into 4 groups: a group with extraction and acute stress (E+A); group with extraction and without stress (E+C); group without extraction and with acute stress (NO+A); and control group without both extraction and stress (NO+C). Masseter muscles were analyzed by Succinate Dehydrogenase (SDH), Nicotinamide Adenine Dinucleotide Diaphorase (NADH) and Reactive Oxygen Species (ROS) techniques. Statistical analyses and two-way ANOVA were applied, followed by Tukey-Kramer tests. In the SDH test, the E+C, E+A and NO+A groups showed a decrease in high desidrogenase activities fibers (P < 0.05), compared to the NO+C group. In the NADH test, there was no difference among the different groups. In the ROS test, in contrast, E+A, E+C and NO+A groups showed a decrease in ROS expression, compared to NO+C groups (P < 0.05). Modified dental occlusion and acute stress - which are important and prevalent problems that affect the general population - are important etiologic factors in metabolic plasticity and ROS levels of masseter muscles. (AU)

• Processo FAPESP: 11/00856-7 - Efeitos do estresse agudo e crônico no músculo masseter em hipofunção mastigatória
• Beneficiário: Mamie Mizusaki Iyomasa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/15209-7 - Efeitos do estresse crônico repetido no músculo masseter em hipofunção mastigatória
• Beneficiário: Ricardo Alexandre Junqueira Calzzani
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/18889-9 - Efeitos do estresse agudo no músculo masseter em hipofunção mastigatória
• Beneficiário: Fernanda Silva Fernandes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica