Clinical, biochemical and histological study of the effect of antimicrobial photodynamic therapy on oral mucositis induced by 5-fluorouracil in hamsters

Autor(es)

da Cruz, Erika de Paula ^[1] ; Campos, Luana ^[1] ; Pereira, Filipi da Silva ^[1] ; Magliano, Gabriela Campos ^[1] ; Benites, Bernar Monteiro ^[1] ; Arana-Chavez, Victor Elias ^[1] ; Ballester, Rafael Yague ^{[1, 2]} ; Simoes, Alyne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Div Dent Mat, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 12, n. 2, p. 298-309, JUN 2015.
• Citações Web of Science: 11

Resumo

Oral mucositis (OM) is a debilitating side effect of chemotherapy, which can be relieved by phototherapy. Antimicrobial photodynamic therapy (aPDT) may be used for the treatment of OM, when infection is present. However, there are no studies showing that aPDT affects tissue repair process when used in the treatment of lesions caused by OM. This work aims to evaluate the effect of aPDT in healing OM induced by 5-Fluorouracil (5-FU). Two hundred forty-five hamsters were divided into two groups, control (C) and experimental, which were subdivided into 4 subgroups (Ch, ChP, ChL, aPDT). C group received only the vehicle of chemotherapy and anesthesia, whereas all animals of the experimental groups received anesthesia and chemotherapy agent 5-FU to induce OM. Ch group received no OM treatment; ChP group received an application of methylene blue (MB) 0.01%; ChL received irradiation with low-power-laser (LPL-660 nm/120J/cm(2)/40mW/4.4J per point); and aPDT received MB and LPL irradiation. OM Clinical severity were daily assessed by a blinded examiner. The animals were sacrificed after 5, 7 and 10 days of experiment and their oral mucosa were removed for biochemical (enzymatic activity of SOD and catalase) and histological analyzes (light microscopy). After statistical analysis was performed, results showed that aPDT reduced the severity of OM on the tenth day of the experiment, when compared to the initial OM score (p< 0.05), as well as increased keratinization with organized collagen deposition in the lamina propria. In conclusion, aPDT can be safely used in animals with infected OM because it does not affect lesion-repairing processes. (c) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/14013-1 - Estudo bioquímico e histológico comparativo do efeito das fototerapias LED, laser de baixa ou alta potência na mucosite oral e do laser na hipofunção das glândulas salivares em hamsters tratados com 5-fluorouracil
• Beneficiário: Alyne Simões Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Regular