Micro-arc oxidation as a tool to develop multifunctional calcium-rich surfaces for dental implant applications

Autor(es):Mostrar menos -

Ribeiro, A. R. ^{[1, 2, 3]} ; Oliveira, F. ^{[1, 4]} ; Boldrini, L. C. ^[3] ; Leite, P. E. ^[3] ; Falagan-Lotsch, P. ^[3] ; Linhares, A. B. R. ^[5] ; Zambuzzi, W. F. ^{[1, 6]} ; Fragneaud, B. ^[7] ; Campos, A. P. C. ^[8] ; Gouvea, C. P. ^[8] ; Archanjo, B. S. ^[8] ; Achete, C. A. ^[8] ; Marcantonio, Jr., E. ^[2] ; Rocha, L. A. ^{[1, 9]} ; Granjeiro, J. M. ^{[1, 3, 10]}

Afiliação do(s) autor(es)

^[1] Brazilian Branch Inst Biomat Tribocorros & Nanome, Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Dept Periodontol, Araraquara Dent Sch, BR-14801903 Sao Paulo - Brazil

^[3] Natl Inst Metrol Qual & Technol, Directory Metrol Appl Life Sci, Rio De Janeiro - Brazil

^[4] Univ Minho, Ctr Mech & Mat Technol, P-4800058 Guimaraes - Portugal

^[5] Univ Fed Fluminense, Clin Res Unit, Antonio Pedro Hosp, Niteroi, RJ - Brazil

^[6] Univ Estadual Paulista, UNESP, Dept Quim & Bioquim, BR-18618970 Botucatu, SP - Brazil

^[7] Fed Univ Juiz de Fora UFJF, Dept Phys, BR-36036900 Juiz De Fora, MG - Brazil

^[8] Natl Inst Metrol Qual & Technol, Div Mat Metrol, Rio De Janeiro - Brazil

^[9] Univ Estadual Paulista, Dept Fis, UNESP, Sao Paulo - Brazil

^[10] Univ Fed Fluminense, Sch Dent, Niteroi, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 54, p. 196-206, SEP 1 2015.
• Citações Web of Science: 31

Resumo

Titanium (Ti) is commonly used in dental implant applications. Surface modification strategies are being followed in last years in order to build Ti oxide-based surfaces that can fulfill, simultaneously, the following requirements: induced cell attachment and adhesion, while providing a superior corrosion and tribocorrosion performance. In this work micro-arc oxidation (MAO) was used as a tool for the growth of a nanostructured bioactive titanium oxide layer aimed to enhance cell attachment and adhesion for dental implant applications. Characterization of the surfaces was performed, in terms of morphology, topography, chemical composition and crystalline structure. Primary human osteoblast adhesion on the developed surfaces was investigated in detail by electronic and atomic force microscopy as well as immunocytochemistry. Also an investigation on the early cytokine production was performed. Results show that a relatively thick hybrid and graded oxide layer was produced on the Ti surface, being constituted by a mixture of anatase, rutile and amorphous phases where calcium (Ca) and phosphorous (P) were incorporated. An outermost nanometric-thick amorphous oxide layer rich in Ca was present in the film. This amorphous layer, rich in Ca, improved fibroblast viability and metabolic activity as well as osteoblast adhesion. High-resolution techniques allowed to understand that osteoblasts adhered less in the crystalline-rich regions while they preferentially adhere and spread over in the Ca-rich amorphous oxide layer. Also, these surfaces induce higher amounts of IFN-gamma, cytokine secretion, which is known to regulate inflammatory responses, bone microarchitecture as well as cytoskeleton reorganization and cellular spreading. These surfaces are promising in the context of dental implants, since they might lead to faster osseointegration. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/03633-1 - Avaliação da biocompatibilidade e do perfil quinômico e de microRNAs de osteoblastos aderidos à superfície modificada de titânio
• Beneficiário: Ana Rosa Lopes Pereira Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado