scFv from Antibody That Mimics gp43 Modulates the Cellular and Humoral Immune Responses during Experimental Paracoccidioidomycosis

Autor(es)

Jannuzzi, Grasielle Pereira ^[1] ; Tavares, Aldo Henrique F. P. ^[2] ; Kaihami, Gilberto Hideo ^[3] ; Fogaca de Almeida, Jose Roberto ^[1] ; de Almeida, Sandro Rogerio ^[1] ; Ferreira, Karen Spadari ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin, Sao Paulo - Brazil

^[2] Univ Brasilia, Inst Biol, Brasilia, DF - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Quim, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Biol, Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 6 JUN 19 2015.
• Citações Web of Science: 2

Resumo

Paracoccidioidomycosis (PCM), caused by Paracoccidioides species is a prevalent systemic and progressive mycosis that occurs in Latin America. It is caused by Paracoccidioides species. Immunization with dendritic cells transfected with a plasmid encoding the scFv (pMAC/PS-scFv) that mimics the main antigen of P. brasiliensis (gp43) confers protection in experimental PCM. DCs link innate and adaptive immunity by recognizing invading pathogens and selecting the type of effector T cell to mediate the immune response. Here, we showed that DC-pMAC/PS-scFv induces the activation of CD4(+) and CD8(+) T cells. Moreover, our results demonstrated that BALB/c mice infected with P. brasiliensis and treated with DC-pMAC/PS-scFv showed the induction of specific IgG production against gp43 and IFN-gamma, IL-12 and IL-4 cytokines. Analysis of regional lymph nodes revealed increases in the expression of clec7a, myd88, tlr2, gata3 and tbx21, which are involved in the immune response. Taken together, our results indicate that the scFv modulates the humoral and cellular immune responses and presents epitopes to CD4(+) and CD8(+) T cells. (AU)

• Processo FAPESP: 13/08548-5 - Estudo dos efeitos da terapia de scFv que mimetiza o antígeno gp43 de Paracoccidioides brasiliensis conjugado à nanocápsulas na paracoccidioidomicose experimental
• Beneficiário: Karen Spadari Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50346-5 - Munofenotipagem e análise da capacidade de ativação de linfócitos TCD4+/CD8+ por células dendríticas transfectada com ScFv obtido a partir de anticorpos monocional anti-idiotípico Ab2-beta, mimetiza o antígeno gp43 de Paracoccidioides brasiliensis
• Beneficiário: Karen Spadari Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular