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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Multi-Analytical Approach Reveals Potential Microbial Indicators in Soil for Sugarcane Model Systems

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Autor(es):
Navarrete, Acacio Aparecido [1] ; Diniz, Tatiana Rosa [1] ; Perez Braga, Lucas Palma [1] ; Silva, Genivaldo Gueiros Zacarias [2] ; Franchini, Julio Cezar [3] ; Rossetto, Raffaella [4] ; Edwards, Robert Alan [5, 6, 2] ; Tsai, Siu Mui [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Cell & Mol Biol Lab, Piracicaba, SP - Brazil
[2] San Diego State Univ, Computat Sci Res Ctr, San Diego, CA 92182 - USA
[3] Brazilian Agr Res Corp, Londrina, PR - Brazil
[4] Sao Paulos Agcy Agribusiness Technol, Piracicaba, SP - Brazil
[5] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 - USA
[6] Argonne Natl Lab, Div Math & Comp Sci, Argonne, IL 60439 - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 10, n. 6 JUN 9 2015.
Citações Web of Science: 21
Resumo

This study focused on the effects of organic and inorganic amendments and straw retention on the microbial biomass (MB) and taxonomic groups of bacteria in sugarcane-cultivated soils in a greenhouse mesocosm experiment monitored for gas emissions and chemical factors. The experiment consisted of combinations of synthetic nitrogen (N), vinasse (V; a liquid waste from ethanol production), and sugarcane-straw blankets. Increases in CO2-C and N2O-N emissions were identified shortly after the addition of both N and V to the soils, thus increasing MB nitrogen (MB-N) and decreasing MB carbon (MB-C) in the N+V-amended soils and altering soil chemical factors that were correlated with the MB. Across 57 soil metagenomic datasets, Actinobacteria (31.5%), Planctomycetes (12.3%), Deltaproteobacteria (12.3%), Alphaproteobacteria (12.0%) and Betaproteobacteria (11.1%) were the most dominant bacterial groups during the experiment. Differences in relative abundance of metagenomic sequences were mainly revealed for Acidobacteria, Actinobacteria, Gammaproteobacteria and Verrucomicrobia with regard to N+V fertilization and straw retention. Differential abundances in bacterial groups were confirmed using 16S rRNA gene-targeted phylum-specific primers for real-time PCR analysis in all soil samples, whose results were in accordance with sequence data, except for Gammaproteobacteria. Actinobacteria were more responsive to straw retention with Rubrobacterales, Bifidobacteriales and Actinomycetales related to the chemical factors of N+V-amended soils. Acidobacteria subgroup 7 and Opitutae, a verrucomicrobial class, were related to the chemical factors of soils without straw retention as a surface blanket. Taken together, the results showed that MB-C and MB-N responded to changes in soil chemical factors and CO2-C and N2O-N emissions, especially for N+V-amended soils. The results also indicated that several taxonomic groups of bacteria, such as Acidobacteria, Actinobacteria and Verrucomicrobia, and their subgroups acted as early-warning indicators of N+V amendments and straw retention in sugarcane-cultivated soils, which can alter the soil chemical factors. (AU)

Processo FAPESP: 11/51749-6 - Bioindicadores funcionais para monitoramento da qualidade do solo para o manejo sustentável da produção de biomassa em cana-de-açúcar (BIOSQ)
Beneficiário:Tsai Siu Mui
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 12/13321-7 - Bio-indicadores microbianos taxonômicos e funcionais em solos sob sistemas de produção de cana-de-açúcar
Beneficiário:Acacio Aparecido Navarrete
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 14/06077-8 - Anotação metagenômica e análise de bioinformática aplicada à definição de bioindicadores microbianos para os efeitos do manejo agrícola do solo em sistemas de produção de cana-de-açúcar
Beneficiário:Acacio Aparecido Navarrete
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado