Fragmentation effects and genetic diversity of the key semidecidual forest species Metrodorea nigra in Southwestern Brazil

Autor(es)

Moraes Filho, R. M. ^{[1, 2]} ; Bonifacio-Anacleto, F. ^[1] ; Alzate-Marin, A. L. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Lab Genet Vegetal, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Programa Posgrad Genet, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 14, n. 2, p. 3509-3524, 2015.
• Citações Web of Science: 4

Resumo

Studies of genetic diversity in plant species present in the remaining fragments of the Atlantic Forest are very important for understanding their resilience to such a degraded ecosystem. We analyzed the genetic diversity of 3 populations of the high-density understory species Metrodorea nigra St. Hill. (Rutaceae) located in forest remnants in the region of Ribeirao Preto, SP, Brazil (M13-Rib, BSQ-Rib, and FAC-Crav), by using simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) molecular markers for conservation purposes. A total of 133 polymorphic loci were observed in 136 inter-simple sequence repeat loci (average of 17 per primer). The Nei genetic diversity (H-E) was relatively high considering all populations (0.31). The BSQ-Rib population exhibited the highest value (0.27), followed by the M13-Rib (0.26) and FAC-Crav (0.24) populations. The simple sequence repeat markers analyzed showed a high number of alleles (K = 104), with an average of 14.85 alleles per locus. The average observed heterozygosity was 0.516 and the average expected heterozygosity was 0.771, ranging from 0.688 (FAC-Crav) to 0.765 (BSQ-Rib). The fixation indexes showed positive and significant differences from zero for all sample sets, indicating inbreeding, which may have resulted from the species' mating patterns and the barochoric seed dispersal system of M. nigra. Both markers indicated differentiation among populations, with higher values observed for inter-simple sequence repeat markers. No significant differences between juvenile and adult generations in any of the fragments were observed, indicating the resilience of M. nigra to the effects of fragmentation and reduced habitat. (AU)

• Processo FAPESP: 13/01265-8 - Estudos genéticos em fina escala de duas espécies arbóreas comuns em remanescentes da Floresta Estacional Semidecidual no interior de SP
• Beneficiário: Ana Lilia Alzate Marin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/18633-0 - IDENTIFICAÇÃO DE MARCADORES ESPECIE-ESPECÍFICOS DE DUAS ESPÉCIES FLORESTAIS DO GENERO Anadenanthera
• Beneficiário: Fernando Bonifácio Anacleto
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico