Enzyme encapsulation in magnetic chitosan-Fe3O4 microparticles

Autor(es)

Costa-Silva, Tales Alexandre ^[1] ; Marques, Polyana Samorano ^[1] ; Fernandes Souza, Claudia Regina ^[1] ; Said, Suraia ^[1] ; Oliveira, Wanderley Pereira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Lab Pharmaceut Proc LAPROFAR, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microencapsulation; v. 32, n. 1, p. 16-21, 2015.
• Citações Web of Science: 3

Resumo

Two simple procedures for the preparation of magnetic chitosan enzyme microparticles have been investigated and used for the immobilisation of endophytic fungus Cercospora kikuchii lipase as model enzyme. In the first case, lipase was entrapped in Fe3O4-chitosan microparticles by cross-linking method, while in the second case magnetic immobilised derivatives were produced using spray drying. Immobilised enzymes showed high enzyme activity retention and stability during storage without significant loss of activity. Glutaraldehyde Fe3O4-chitosan powders presented a higher lipase activity retention and storage stability than the others preparations. However, the immobilised derivatives produced by cross-linking showed higher enzyme activity after reuse cycles. The results proved that the magnetic Fe3O4-chitosan microparticles are an effective support for the enzyme immobilisation since the immobilised lipase showed best properties than the free form. (AU)

• Processo FAPESP: 11/00743-8 - Produção e imobilização de lipases produzidas pelo fungo endofítico Cercospora kikuchii para aplicações biotecnológicas
• Beneficiário: Tales Alexandre da Costa e Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado