Evaluation of Potential Thrombin Inhibitors from the White Mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)

Autor(es)

Bittencourt Rodrigues, Caroline Fabri ^[1] ; Gaeta, Henrique Hessel ^[1] ; Belchor, Mariana Novo ^[1] ; Pena Ferreira, Marcelo Jose ^[2] ; Terashima Pinho, Marcus Vinicius ^[1] ; Toyama, Daniela de Oliveira ^[3] ; Toyama, Marcos Hikari ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, BR-11330900 Sao Vicente, SP - Brazil

^[2] Univ Sao Paulo, Dept Bot, Inst Biociencias, BR-05508090 Sao Paulo, SP - Brazil

^[3] Unicastelo Univ Camilo Castelo Branco, BR-08230030 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE DRUGS; v. 13, n. 7, p. 4505-4519, JUL 2015.
• Citações Web of Science: 2

Resumo

The aim of this work was to verify the effects of methanol (MeOH) and hydroalcoholic (HA) extracts and their respective partition phases obtained from white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.) leaves on human thrombin activity. Among the extracts and phases tested, only the ethyl acetate and butanolic partitions significantly inhibited human thrombin activity and the coagulation of plasma in the presence of this enzyme. Chromatographic analyses of the thrombin samples incubated with these phases revealed that different compounds were able to interact with thrombin. The butanolic phase of the MeOH extract had the most potent inhibitory effects, reducing enzymatic activity and thrombin-induced plasma coagulation. Two glycosylated flavonoids in this partition were identified as the most potent inhibitors of human thrombin activity, namely quercetin-3-O-arabinoside (QAra) and quercetin-3-O-rhamnoside (Qn). Chromatographic analyses of thrombin samples incubated with these flavonoids demonstrated the chemical modification of this enzyme, suggesting that the MeOH extract contained other compounds that both induced structural changes in thrombin and diminished its activity. In this article, we show that despite the near absence of the medical use of mangrove compounds, this plant contains natural compounds with potential therapeutic applications. (AU)

• Processo FAPESP: 13/10938-6 - Investigação de polissacarídeos sulfatados de baixo peso molecular de macroalgas marinhas sobre a estrutura e função fosfolipase A2 secretória de Crotalus durissus terrificus
• Beneficiário: Marcos Hikari Toyama
• Modalidade de apoio: Auxílio à Pesquisa - Regular