Osteoblast response to porous titanium and biomimetic surface: In vitro analysis

Autor(es)

do Prado, Renata Falchete ^[1] ; de Oliveira, Femanda Saraiva ^[1] ; Nascimento, Rodrigo Dias ^{[2, 3]} ; Reis de Vasconcellos, Luana Marotta ^[1] ; Carvalho, Yasmin Rodarte ^[1] ; Alves Cairo, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Sao Jose Campos SP, Dept Biosci & Oral Diag, Inst Sci & Technol, Sch Dent, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Sao Jose Campos SP, Dept Diag, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Sao Jose Campos SP, Surg Inst Sci & Technol, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[4] CTA, Div Mat, Air & Space Inst, BR-12904000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 52, p. 194-203, JUL 1 2015.
• Citações Web of Science: 15

Resumo

Objective: This study analyzed the behavior of human osteoblasts cultured on porous titanium specimens, with and without biomimetic treatment, compared to dense titanium. Design: The experiment had seven groups: Group 1: cells cultured on polystyrene of culture plate wells; Group 2: cells cultured on dense titanium specimen; Group 3: specimen with 33.79% of pores; Group 4: 41.79% of pores; Groups 5, 6 and 7: specimens similar to groups 2,3 and 4, yet with biomimetic treatment Real time-polymerase chain reaction with reverse transcription of the following genes was performed: prostaglandin E2 synthase, integrin beta 1, osterix, Runx2, Interleukin 6, macrophage colony stimulating factor, apolipoprotein E and others. The study achieved data on cell adhesion, growth and viability, total protein content, alkaline phosphatase activity and quantity of mineralized nodule formations. Data were statistically evaluated. Results: Adherent cells and alkaline phosphatase activity were similar in titanium specimens, regardless of the groups. Biomimetic treatment reduced the total protein activity and the viability of tested cells. Most tested genes had statistically similar expression in all groups. Conclusion: The tested porosities did not cause alterations in osteoblast behavior and the biomimetic treatment impaired the biocompatibility of titanium causing cytotoxicity. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/19938-3 - Implantes porosos de titânio e tratamento biomimético: efeitos na expressão gênica de osteoblastos derivados de osso alveolar humano
• Beneficiário: Carlos Alberto Alves Cairo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/02778-0 - Implantes porosos de titânio e tratamento biomimético: Efeitos na expressão gênica de osteoblastos derivados osso alveolar humano
• Beneficiário: Renata Falchete Do Prado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado