Mesophilic hydrogen production in acidogenic packed-bed reactors (APBR) using raw sugarcane vinasse as substrate: Influence of support materials

Autor(es)

Nunes Ferraz Junior, Antonio Djalma ^{[1, 2]} ; Etchebehere, Claudia ^[3] ; Zaiat, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Ctr Res Dev & Innovat Environm Engn, Biol Proc Lab, BR-13563120 Sao Carlos, SP - Brazil

^[2] CTBE Brazilian Bioethanol Sci & Technol Lab CNPEM, BR-13083970 Campinas, SP - Brazil

^[3] Inst Invest Biol Clemente Estable, Lab Ecol Microbiana, Montevideo - Uruguay

• Tipo de documento: Artigo Científico
• Fonte: Anaerobe; v. 34, p. 94-105, AUG 2015.
• Citações Web of Science: 18

Resumo

Bio-hydrogen production from sugarcane vinasse in anaerobic up-flow packed-bed reactors (APBR) was evaluated. Four types of support materials, expanded clay (EC), charcoal (Ch), porous ceramic (PC), and low-density polyethylene (LDP) were tested as support for biomass attachment. APBR (working volume -2.3 L) were operated in parallel at a hydraulic retention time of 24 h, an organic loading rate of 36.2 kg-COD m(-3) d(-1), at 25 degrees C. Maximum volumetric hydrogen production values of 509.5, 404, 81.4 and 10.3 mL-H-2 d(-1) L-reactor(-1) and maximum yields of 3.2, 2.6, 0.4 and 0.05 mol-H-2 mol(-1) carbohydrates total, were observed during the monitoring of the reactors filled with LDP, EC, Ch and PC, respectively. Thus, indicating the strong influence of the support material on H-2 production. LDP was the most appropriate material for hydrogen production among the materials evaluated. 16S rRNA gene by Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and scanning electron microscopy confirmed the selection of different microbial populations. 454-pyrosequencing performed on samples from APBR filled with LDP revealed the presence of hydrogen-producing organisms (Clostridium and Pectinatus), lactic acid bacteria and non-fermentative organisms. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/15984-0 - Produção de bioenergia no tratamento de águas residuárias e adequação ambiental dos efluentes e resíduos gerados
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/15665-8 - Estrutura e dinâmica funcional da comunidade microbiana de digestores anaeróbios, aplicados à recuperação de energia e adequação ambiental de resíduos gerados no processo produtivo do bioetanol de 1ª e 2ª geração
• Beneficiário: Antônio Djalma Nunes Ferraz Júnior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado