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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Understanding the biocide action of poly(hexamethylene biguanide) using Langmuir monolayers of dipalmitoyl phosphatidylglycerol

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Autor(es):
Souza, Adriano L. [1] ; Ceridorio, Lucineia F. [2] ; Paula, Gustavo F. [3] ; Mattoso, Luiz H. C. [3] ; Oliveira, Jr., Osvaldo N. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Carlos, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci, BR-09972270 Diadema, SP - Brazil
[3] Natl Nanotechnol Lab Agribusiness LNNA, Embrapa Instrumentat, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 132, p. 117-121, AUG 1 2015.
Citações Web of Science: 6
Resumo

The disinfectant activity of poly(hexamethylene biguanide) (PHMB) has been explored in industrial applications, in agriculture and in food manipulation, but this biocide action is not completely understood. It is believed to arise from electrostatic interactions between the polyhexanide group and phosphatidylglycerol, which is the main phospholipid on the bacterial membrane. In this study, we investigated the molecular-level interactions between PHMB and dipalmitoyl phosphatidylglycerol (DPPG) in Langmuir monolayers that served as cell membrane models. PHMB at a concentration of 2 x 10(-4) g L-1 in a Theorell-Stenhagen at pH 3.0 and in a phosphate at pH 7.4 was used as a subphase to prepare the DPPG monolayers. Surface pressure-area isotherms showed that PHMB adsorbs and penetrates into the DPPG monolayers, expanding them and increasing their elasticity under both conditions examined. Results from polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) indicated that PHMB induces disorder in the DPPG chains and dehydrates their C=O groups, especially for the physiological medium. Overall, these findings point to hydrophobic interactions and dehydration being as relevant as electrostatic interactions to explain changes in membrane fluidity and permeability, believed to be responsible for the biocide action of PHMB. (c) 2015 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
Beneficiário:Osvaldo Novais de Oliveira Junior
Modalidade de apoio: Auxílio à Pesquisa - Temático