Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source

Autor(es)

Sia, Eliandra de Freitas ^[1] ; Marcon, Joelma ^[2] ; Luvizotto, Danice Mazzer ^[2] ; Quecine, Maria Carolina ^[2] ; Tsui, Sarina ^[2] ; Pereira, Jose Odair ^[1] ; Pizzirani-Kleiner, Aline Aparecida ^[2] ; Azevedo, Joao Lucio ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Amazonas UFAM, BR-69077000 Manaus, Amazonas - Brazil

^[2] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPRINGERPLUS; v. 2, 2013.
• Citações Web of Science: 5

Resumo

Endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. Fungal growth is a routine practice at microbiological laboratories, and the Potato Dextrose Agar (PDA) is the most frequently used medium because it is a rich source of starch. However, the production of potatoes in some regions of the world can be costly. Aiming the development of a new medium source to tropical countries, in the present study, we used leaves from the guarana (a tropical plant from the Amazon region) and the olive (which grows in subtropical and temperate regions) to isolate endophytic fungi using PDA and Manihot Dextrose Agar (MDA). Cassava (Manihot esculenta) was evaluated as a substitute starch source. For guarana, the endophytic incidence (EI) was 90% and 98% on PDA and MDA media, respectively, and 65% and 70% for olive, respectively. The fungal isolates were sequenced using the ITS-rDNA region. The fungal identification demonstrated that the isolates varied according to the host plant and media source. In the guarana plant, 13 fungal genera were found using MDA and six were found using PDA. In the olive plant, six genera were obtained using PDA and 4 were obtained using MDA. The multivariate analysis results demonstrated the highest fungal diversity from guarana when using MDA medium. Interestingly, some genera were isolated from one specific host or in one specific media, suggesting the importance of these two factors in fungal isolation specificity. Thus, this study indicated that cassava is a feasible starch source that could serve as a potential alternative medium to potato medium. (AU)

• Processo FAPESP: 10/50445-0 - Estado da variabilidade genética de Puccinia psidii Winter, agente causal de ferrugem em Eucalyptus spp.
• Beneficiário: Maria Carolina Quecine Verdi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/15192-4 - Estudo da diversidade de bactérias endofíticas cultiváveis associadas ao guaranazeiro [Paullinia cupana (Mart.) Ducke.] visando o controle de antracnose
• Beneficiário: Sarina Tsui
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/53376-2 - Microrganismos associados ao guaranazeiro com potencial biotecnológico e agrícola, especialmente visando o controle da antracnose
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/18740-5 - Detecção e Caracterização de Quitinases em bibliotecas metagenomicas de Manguezais do Estado de São Paulo.
• Beneficiário: Joelma Marcon
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado