Bioprospecting endophytic fungi isolated from Amazon guarana tree
| Full text | |
| Author(s): |
Sia, Eliandra de Freitas
[1]
;
Marcon, Joelma
[2]
;
Luvizotto, Danice Mazzer
[2]
;
Quecine, Maria Carolina
[2]
;
Tsui, Sarina
[2]
;
Pereira, Jose Odair
[1]
;
Pizzirani-Kleiner, Aline Aparecida
[2]
;
Azevedo, Joao Lucio
[2, 1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Amazonas UFAM, BR-69077000 Manaus, Amazonas - Brazil
[2] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | SPRINGERPLUS; v. 2, 2013. |
| Web of Science Citations: | 5 |
| Abstract | |
Endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. Fungal growth is a routine practice at microbiological laboratories, and the Potato Dextrose Agar (PDA) is the most frequently used medium because it is a rich source of starch. However, the production of potatoes in some regions of the world can be costly. Aiming the development of a new medium source to tropical countries, in the present study, we used leaves from the guarana (a tropical plant from the Amazon region) and the olive (which grows in subtropical and temperate regions) to isolate endophytic fungi using PDA and Manihot Dextrose Agar (MDA). Cassava (Manihot esculenta) was evaluated as a substitute starch source. For guarana, the endophytic incidence (EI) was 90% and 98% on PDA and MDA media, respectively, and 65% and 70% for olive, respectively. The fungal isolates were sequenced using the ITS-rDNA region. The fungal identification demonstrated that the isolates varied according to the host plant and media source. In the guarana plant, 13 fungal genera were found using MDA and six were found using PDA. In the olive plant, six genera were obtained using PDA and 4 were obtained using MDA. The multivariate analysis results demonstrated the highest fungal diversity from guarana when using MDA medium. Interestingly, some genera were isolated from one specific host or in one specific media, suggesting the importance of these two factors in fungal isolation specificity. Thus, this study indicated that cassava is a feasible starch source that could serve as a potential alternative medium to potato medium. (AU) | |
| FAPESP's process: | 10/15192-4 - Study of the diversity of culturable endophytic bacteria associated with guarana [Paullinia cupana (Mart.) ducke.] for the control of anthracnose |
| Grantee: | Sarina Tsui |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 11/18740-5 - Detection and characterization of chitinases in metagenomics libraries from mangrove located in the State of São Paulo |
| Grantee: | Joelma Marcon |
| Support type: | Scholarships in Brazil - Post-Doctorate |